Size??100ug
Cat code??200797
Species Cross-reactivity??H
Key Application??WB
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG2b
Molecular Weight??260kDa
Sensitivity??This antibody detects endogenous levels of CHD4 and does not cross-react with related proteins.
Immunogen??Purified recombinant human CHD4 protein fragments expressed in E.coli.
UniProt Number??Q14839
GeneBank ID??NM_001273
Subcellular location??Nucleus.Cytoplasm cytoskeleton centrosome.
Formulation??Purified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,50% glycerol.
Altername??ATP-dependent helicase CHD4,Mi-2 autoantigen 218 kDa protein,Mi2-beta
Gene_symbol??Mi-2b;Mi2-BETA
Summary??The product of this gene belongs to the SNF2/RAD54 helicase family.It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression.Patients with dermatomyositis develop antibodies against this protein.[provided by RefSeq,Jul 2008]
UniPort summary  Function??Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.
Application Images :??
Western blot detection of CHD4 in K562,HelaNE,HL-60,MOLT-4,Jurkat & 293 cell lysates using CHD4 antibody(1:1000 diluted).Predicted band size:260KDa,Observed band size:260KDa.