Product: Anti-EpCAM mAb, clone VU-1D9
Cat. No.: MC-987 (100 μg)
Background:
Epithelial cell adhesion molecule (EpCAM), also
known as epithelial-specific antigen and EGP-40,
is a homophilic Ca2+-independent cell adhesion
molecule expressed on the basolateral surfaces
of most epithelial cells. Cells of nervous,
muscular and stromal tissue do not express
EpCAM. EpCAM expression is also absent on
mesothelial cells and mesotheliomas, and thus
is a suitable marker for discriminating between
mesothelioma and carcinoma cells. In the
mammary gland, all cells within the mammary
epithelium except for the myoepithelial cells
express EpCAM.
Specificity:
Reacts with EpCAM.
Species Reactivity:
Human. Others not tested.
Ig Isotype:
Mouse IgG1
Immunogen:
The VU-1D9 clone was generated immunizing a
BALB/c mouse with NCl-H69 small cell lung
carcinoma cells. Splenocytes were fused with
SP2/0 myeloma cells.
Format:
100 μg of monoclonal antibody in phosphate
buffered saline (PBS).
Storage:
Store at 4°C. Do not freeze.
Applications and Suggested Dilutions:

Flow cytometry: Recommend 106 cells in 100
μL and 1-3 μg/mL antibody.

Immunohistochemistry: Paraffin sections: Use
at 1-3 μg/mL; Heat-induced antigen retrieval
protocol is required for optimal staining of
formalin-fixed paraffin-embedded tissues.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.