6-Phosphofructokinase Activity Assay試劑盒(Colorimetric)
產(chǎn)品名稱： 6-Phosphofructokinase Activity Assay試劑盒(Colorimetric)
英文名稱： 6-Phosphofructokinase Activity Assay試劑盒(Colorimetric)
產(chǎn)品編號： ab155898
產(chǎn)品價格： null
產(chǎn)品產(chǎn)地： 英國
品牌商標： abcam
更新時間： null
使用范圍：
- 聯(lián)系人 :
- 地址 : 深圳市寶安區(qū)西鄉(xiāng)寶民二路賢基大廈4E
- 郵編 :
- 所在區(qū)域 : 廣東
- 電話 : 133****4454 點擊查看
- 傳真 : 點擊查看
- 郵箱 : 1484332550@qq.com
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- 樣品類型
Adherent cells, Suspension cells, Tissue Homogenate
- 檢測類型Enzyme activity
- 靈敏度
< 1 mU/well
- 范圍
2 nmol/well - 10 nmol/well
- 檢測時間
1h 30m
- 種屬反應(yīng)性
與反應(yīng):?Mouse, Rat, Human
預(yù)測可用于:?all Mammals
- 產(chǎn)品概述
In Abcam's?6-Phosphofructokinase Activity Assay kit (ab155898), PFK (phosphofructokinase) converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. PFK activity assay is simple, robust, and rapid and can detect phosphofructokinase activity less than 1 mU.
- 說明
Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet). In humans, PFK deficiency causes glycogen storage disease, also called Tarui’s disease, which is characterized by exercise-induced muscle weakness and cramps. On the other hand, increased PFK activity contributes to cancer cell proliferation and tumorigenicity. Early detection of abnormal phosphofructokinase activity is crucial for diagnosis, prediction and therapeutic strategy.
- 經(jīng)測試應(yīng)用Functional Studiesmore details
性能
- 存放說明Store at -20°C. Please refer to protocols.
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組件 標識符 100 tests ATP Orange 1 vial NADH Standard Yellow 1 vial PFK Assay Buffer WM 1 x 27ml PFK Developer Red 1 vial PFK Enzyme Mix Green 1 vial PFK Positive Control Purple 1 vial PFK Substrate Blue 1 vial - 研究領(lǐng)域
應(yīng)用
Our?Abpromise guarantee?covers the use of?ab155898?in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
6-Phosphofructokinase Activity Assay Kit (Colorimetric) 圖像
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Phosphofructokinase activity using ab155898