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?英文名稱GFAP
中文名稱膠質纖維酸性蛋白單克隆抗體
別? ? 名Astrocyte; FLJ45472; GFAP; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN.??
研究領域腫瘤? 細胞生物? 神經生物學??
抗體來源Mouse
克隆類型Monoclonal
克 隆 號7D8
交叉反應Mouse, Rat,?
產品應用WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 （石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量49kDa
細胞定位細胞漿?
性? ? 狀Liquid
濃? ? 度1mg/ml
免 疫 原Recombinant mouse GFAP full length:?
亞? ? 型IgG
純化方法affinity purified by Protein G
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產品介紹This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
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Function:
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
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Subunit:
Interacts with SYNM. Isoform 3 interacts with PSEN1 (via N-terminus).
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Subcellular Location:
Cytoplasm. Note=Associated with intermediate filaments.
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Tissue Specificity:
Expressed in cells lacking fibronectin.
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Post-translational modifications:
Phosphorylated by PKN1.
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DISEASE:
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
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Similarity:
Belongs to the intermediate filament family.
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SWISS:
P14136
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Gene ID:
2670
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Database links:
Entrez Gene: 281189 Cow
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Entrez Gene: 2670 Human
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Entrez Gene: 14580 Mouse
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Entrez Gene: 24387 Rat
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Omim: 137780 Human
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SwissProt: Q28115 Cow
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SwissProt: P14136 Human
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SwissProt: P03995 Mouse
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Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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星形膠質細胞標志物 （Astrocyte Marker）
GFAP是一個56kDa的中間絲蛋白（intermediate filament，IF），在中樞神經系統發育期是一個特異性的標志物，以區別星形細胞和其它膠質細胞。GFAP表達在皮層和海馬,急、慢性皮質酮治療時表達減少。
GFAP可以和人、大鼠、小鼠的GFAP反應，在正常和腫瘤性的星形膠質細胞陽性表達，而神經節細胞、神經元、成纖維細胞、少突膠質細胞和這些細胞來源的腫瘤細胞陰性表達，主要用于星形膠質瘤等中樞神經系統腫瘤的診斷和鑒別診斷,GFAP的缺乏可導致AD病。
產品圖片
Sample:
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti- GFAP (bsm-33065M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Spinal cord (Mouse) Lysate at 40 ug
Primary: Anti- TBX1 (bsm-33065M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample: mouse brain Lysate at 25 ug
Primary: Mouse Anti-GFAP(bsm-33065M) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 49kD
Observed band size: 49kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:800 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:400 and 1:800 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:800 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Tissue/cell: BV-2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-33065M) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody (bs-0296G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.
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