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The kit mainly includes three tubes of reagents: Enzyme Mix containing DNase, RNase inhibitor and reverse transcriptase; 5× EZscript All-in-one RT Buffer, containing buffer, dNTPs, Oligo dT18 and Random Hexamer, etc; NC Buffer.

The reverse transcriptase is a genetic engineered enzyme based on M-MLV (RNase H-) reverse transcriptase. And the multiple site-mutations of the reverse transcriptase can obviously increase its affinity to RNA templates and its strand extending ability, which make reverse transcription reaction more efficient. Moreover, this transcriptase is rather resistant to common reverse transcriptase inhibitors. At the same time, the kit uses the latest optimized reaction system to further improve the reverse transcription efficiency. The reverse transcription reaction products are recommended for PCR and qPCR.
Features:
1. Fast and simple procedure: genomic DNA removal and reverse transcription reaction is carried out in one step.
2. The NC Buffer does not contain reverse transcriptase, which can be used to detect whether there is genomic DNA residue in RNA template.
3. Excellent reverse transcription efficiency: Compared with common commercial reverse transcription products, this kit reverses cDNA with the smallest quantitative CT value and has excellent reverse transcription efficiency.
4. Extensive template compatibility: Compatible with RNA templates of different species and poor integrity.