?英文名稱HSP90 beta
中文名稱熱休克蛋白90β/HSP90 β 單克隆抗體
別? ? 名HSP90 beta; D6S182; FLJ26984; Heat shock 90kD protein 1, beta; Heat shock protein 90kDa alpha (cytosolic) class B member 1; Heat shock protein beta; Heat shock protein HSP 90 beta; HSP 84; HSP84; HSP 90; HSP-90 BETA; HSP90B; HSPC2; HSPCB; HS90B_HUMAN; Heat shock protein HSP 90-beta; Heat shock 84 kDa.??
研究領域腫瘤? 免疫學? 信號轉導? 轉錄調節因子??
抗體來源Mouse
克隆類型Monoclonal
克 隆 號2A2
交叉反應Human, Mouse, Rat,?
產品應用WB=1:500-1000?
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量84kDa
細胞定位細胞漿?
性? ? 狀Liquid
濃? ? 度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human HSP90 beta:?
亞? ? 型IgG
純化方法affinity purified by Protein G
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產品介紹This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012].
?
Function:
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
?
Subunit:
Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4.
?
Subcellular Location:
Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
?
Post-translational modifications:
Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity (Probable).
?
Similarity:
Belongs to the heat shock protein 90 family.
?
SWISS:
P08238
?
Gene ID:
3326
?
Database links:
Entrez Gene: 3326 Human
?
Entrez Gene: 15516 Mouse
?
Entrez Gene: 301252 Rat
?
Omim: 140572 Human
?
SwissProt: P08238 Human
?
SwissProt: P11499 Mouse
?
SwissProt: P34058 Rat
?
Unigene: 509736 Human
?
Unigene: 2180 Mouse
?
Unigene: 98667 Rat?
?
?
?
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
?
HSP是細胞受應激原刺激后誘導產生的一組應激蛋白，與腫瘤發生、增殖及分化有關，HSP90β能夠抑制細胞凋亡與分化。
產品圖片
Sample:
Hela KO HSP90 beta(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-HSP90 beta (bsm-33136M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse t 1/20000 dilution
Predicted band size: 84 kD
Observed band size: 84 kD
Sample:
K562 (Human) Lysate at 30 ug
Primary: Anti- HSP90 beta (bsm-33136M) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84 kD
Observed band size: 84 kD
Sample:
Hela (Human) Lysate at 30 ug
Primary: Anti- HSP90 beta (bsm-33136M) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84 kD
Observed band size: 84 kD
Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 4: K562 (Human) Cell Lysate at 30 ug
Lane 5: HepG2 (Human) Cell Lysate at 30 ug
Lane 6: U2os (Human) Cell Lysate at 30 ug
Lane 7: A549 (Human) Cell Lysate at 30 ug
Lane 8: HL60 (Human) Cell Lysate at 30 ug
Lane 9: Jurkat (Human) Cell Lysate at 30 ug
Lane 10: Testis (Mouse) Lysate at 40 ug
Lane 11: Testis (Rat) Lysate at 40 ug
Lane 12: Cerebrum (Mouse) Lysate at 40 ug
Lane 13: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-HSP90 beta (bsm-33136M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 90 kD
Observed band size: 86 kD
Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 beta) Monoclonal Antibody, Unconjugated (ascites of bsm-33136M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSP90 beta) Monoclonal Antibody, Unconjugated (ascites of bsm-33136M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
?
?