Size??100ug
Cat code??310004
Species Cross-reactivity??H,Mk
Key Application??WB
Host??Rabbit
Antibody type??Polyclonal antibody
Purified method??Affinity purified
Isotype??
Molecular Weight??26kDa
Sensitivity??The antibody detects endogenous level of total BCL-2 protein.
Immunogen??Peptide sequence around aa. 54~58 (G-H-T-P-H) derived from Human BCL-2.
UniProt Number??P10415
GeneBank ID??NM_000633.2
Subcellular location??Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane;
Formulation??Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Altername??Apoptosis regulator Bcl 2 ;Apoptosis regulator Bcl-2 ;Apoptosis regulator Bcl2 ; AW986256 ;B cell CLL/lymphoma 2 ;B cell leukemia/lymphoma 2 ;B cell lymphoma 2 ;Bcl 2 ;Bcl-2 ;Bcl2 ;BCL2 protein ;BCL2_HUMAN ;C430015F12Rik ;D630044D05Rik ;D830018M01Rik ;Leukemia/lymphoma, B-cell, 2 ;Oncogene B-cell leukemia 2 ;PPP1R50 ;Protein phosphatase 1, regulatory subunit 50 .
Gene_symbol??Bcl-2; PPP1R50
Summary??This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]
UniPort summary  Function??Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
Application Images :??
Western blot detection of BCL2 antibody in a549, Hela and COS7 cell lysates using BCL2 antibody(dilution 1:300).Predicted band size:26 Kda.Observed band size:26KDa.