DAAM1 (N-term) Peptide
產(chǎn)品名稱： DAAM1 (N-term) Peptide
英文名稱： DAAM1 (N-term) Peptide
產(chǎn)品編號： P1207
產(chǎn)品價(jià)格： 0
產(chǎn)品產(chǎn)地： 臺灣
品牌商標(biāo)： Abnova
更新時(shí)間： null
使用范圍： null
亞諾法生技股份有限公司（Abnova)
- 聯(lián)系人 :
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- 郵編 : 11493
- 所在區(qū)域 : 臺灣
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- 郵箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of DAAM1.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human DAAM1.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein A purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Mechanism of activation of the Formin protein Daam1.
Liu W, Sato A, Khadka D, Bharti R, Diaz H, Runnels LW, Habas R.Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):210-5. Epub 2007 Dec 27.
- 2.
- Crystal structure of human DAAM1 formin homology 2 domain.
Yamashita M, Higashi T, Suetsugu S, Sato Y, Ikeda T, Shirakawa R, Kita T, Takenawa T, Horiuchi H, Fukai S, Nureki O.Genes Cells. 2007 Nov;12(11):1255-65.
- 3.
- Structure of the FH2 domain of Daam1: implications for formin regulation of actin assembly.
Lu J, Meng W, Poy F, Maiti S, Goode BL, Eck MJ.J Mol Biol. 2007 Jun 22;369(5):1258-69. Epub 2007 Apr 5.
- Applications
- Western Blot (Transfected lysate)
- Western blot analysis of DAAM1 (arrow) using DAAM1 polyclonal antibody (Cat # PAB1951). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DAAM1 gene (Lane 2) (Origene Technologies).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human brain tissue reacted with DAAM1 polyclonal antibody (Cat # PAB1951) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Entrez GeneID:
- 23002
- Protein Accession#:
- NP_055807;Q9Y4D1
- Gene Name:
- DAAM1
- Gene Alias:
- FLJ41657,KIAA0666
- Gene Description:
- dishevelled associated activator of morphogenesis 1
- Omim ID:
- 606626
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Functions of the cell cortex, including motility, adhesion, and cytokinesis, are mediated by the reorganization of the actin cytoskeleton and recent evidence suggests a role for the Formin homology (FH) proteins in these processes. The protein encoded by this gene contains FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function as a scaffolding protein. Evidence of alternative splicing has been observed for this gene but the full-length nature of these variants has not been determined. [provided by RefSeq
- Other Designations:
- OTTHUMP00000179033,dishevelled-associated activator of morphogenesis 1
- Gene Pathway
- Related Disease