Product: Anti-Bid (CT), Polyclonal
Cat. No.: PC-474 (100 μg)
Description:
Apoptosis plays a major role in normal organism
development, tissue homeostasis, and removal
of damaged cells. Disruption of this process has
been implicated in a variety of diseases such as
cancer. The Bcl-2 family of proteins is comprised
of critical regulators of apoptosis that can be
divided into two classes: those that inhibit
apoptosis and those that promote cell death. Bid,
a pro-apoptotic Bcl-2 family member, is cleaved
by caspase-8 in response to apoptotic signals,
exposing the Bcl-2 homology 3 (BH3) domain
which is normally buried in the full-length protein.
The cleaved complex is myristoylated and
translocated to the mitochondrial membrane
where it may induce mitochondrial Bax and Bak
to oligomerize.
Specificity:
Recognizes Bid.
Species Reactivity:
Human and mouse. Other species not tested.
Host: Rabbit
Immunogen:
Synthetic peptide corresponding to 14 amino
acids near the carboxyl-terminus of human Bid
(Genbank accession No. AAH36364).
Format:
100 μg in 100 μl of PBS containing 0.02%
sodium azide. IgG purified by ion exchange
chromatography.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Western blot: Recommend 0.5 – 1.0 μg/ml.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.