Size??100ug
Cat code??200789
Species Cross-reactivity??H
Key Application??WB
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG2a
Molecular Weight??72kDa
Sensitivity??This antibody detects endogenous levels of NRF1,and does not cross-react with related proteins.
Immunogen??Purified recombinant NRF1 protein fragments expressed in E.coli
UniProt Number??Q16656
GeneBank ID??NM_001040110
Subcellular location??Nucleus.
Formulation??Purified mouse monoclonal in PBS(pH 7.4)containing with 0.2% sodium azide,50% glycerol,0.1%BSA.
Altername??alpha pal;alpha palindromic binding protein;Alpha-pal;locus control region factor 1;NFE2 related factor 1;NRF-1;Nrf1;nuclear factor (erythroid derived 2)-like 1;nuclear respiratory factor 1;transcription factor 11;
Gene_symbol??ALPHA-PAL
Summary??This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration,heme biosynthesis,and mitochondrial DNA transcription and replication.The protein has also been associated with the regulation of neurite outgrowth.Alternate transcriptional splice variants,which encode the same protein, have been characterized.Additional variants encoding different protein isoforms have been described but they have not been fully characterized.Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for ""nuclear factor(erythroid-derived 2)-like 1"" which has an official symbol of NFE2L1.[provided by RefSeq, Jul 2008]"
UniPort summary  Function??Transcription factor that activates the expression of the EIF2S1(EIF2-alpha)gene.Links the transcriptional modulation of key metabolic genes to cellular growth and development.Implicated in the control of nuclear genes required for respiration,heme biosynthesis,and mitochondrial DNA transcription and replication.
Application Images :??
Western blot detection of NRF1 in Hela,K562,CEM&U2OS cell lysates using NRF1 antibody(1:1000 diluted).Predicted band size:67KDa,Observed band size:72KDa