Beclin-1 (2A4) Mouse mAb
產品名稱： Beclin-1 (2A4) Mouse mAb
英文名稱： Beclin-1 (2A4) Mouse mAb
產品編號： #4122
產品價格： 0
產品產地： 美國
品牌商標： CST
更新時間： 2023-09-19T21:32:30
使用范圍： null
- 聯系人 :
- 地址 : 杭州市西溪路525號浙江大學科技園B座501室
- 郵編 : 310029
- 所在區域 : 浙江
- 電話 : 137****2380 點擊查看
- 傳真 : 點擊查看
- 郵箱 : service@ws-bio.com
?
?
Beclin-1 (2A4) Mouse mAb #4122
| No. | Size | ? |
|---|---|---|
| 4122S | 100 ul ( 10 western blots ) | ? |
| 4122 | carrier free & custom formulation / quantity | ? |
| Application | Dilution | Species-Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|---|
| W | 1:1000 | Human | Endogenous | 60 | Mouse |
| IP | 1:50 |
Species cross-reactivity is determined by western blot.
Applications Key:?W=Western Blotting,?IP=Immunoprecipitation
Specificity / Sensitivity
Beclin-1 (2A4) Mouse mAb recognizes endogenous levels of total human Beclin-1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to a central region within human Beclin-1 protein.
Western Blotting
Western blot analysis of extracts from various cell lines using Beclin-1 (2A4) Mouse mAb.
Background
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions (1). One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30 (2). Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells (3). Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL, but not with Bax or Bak (4). While Beclin-1 is generally ubiquitously expressed, research studies have shown it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers (5). Beclin-1 is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export (6). Investigators have demonstrated that Beclin-1 -/- mice die early in embryogenesis and Beclin-1 -/+ mice have a high incidence of spontaneous tumors. Stem cells from the null mice demonstrate an altered autophagic response, although responses to apoptosis appeared normal (7). Researchers have also found that overexpression of Beclin-1 in virally infected neurons?in vivo?resulted in significant protection against Sindbis virus-induced disease and neuronal apoptosis (4).
- Reggiori, F. and Klionsky, D.J. (2002)?Eukaryot Cell?1, 11-21.
- Kametaka, S. et al. (1998)?J Biol Chem?273, 22284-91.
- Liang, X.H. et al. (1999)?Nature?402, 672-6.
- Liang, X.H. et al. (1998)?J Virol?72, 8586-96.
- Aita, V.M. et al. (1999)?Genomics?59, 59-65.
- Liang, X.H. et al. (2001)?Cancer Res?61, 3443-9.
- Yue, Z. et al. (2003)?Proc Natl Acad Sci USA?100, 15077-82.
Application References
Have?you?published research involving the use of our products? If so wed love to hear about it. Please?let us know!
Companion Products
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology?is a trademark of Cell Signaling Technology, Inc.
?