Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco DMEM modifications for a range of applications. Find the right modification using the media selector tool.

This DMEM is modified as follows:
With Without
   Glucose
   Phenol red
   L-arginine
   L-glutamine
   L-lysine
   Sodium pyruvate
   HEPES

DMEM is unique from other media as it contains 4 times the concentrations of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. SILAC DMEM Flex has been further modified to not contain lysine, arginine, and glutamine. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). SILAC protein labeling experiments should be conducted using Gibco Dialyzed FBS. DMEM uses a sodium bicarbonate buffer system (3.7 g/L), so requires a 5-10% CO2 environment to maintain physiological pH.

cGMP Manufacturing and Quality System：
SILAC DMEM Flex is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.