Anti-Human IP-10
Catalog Number:
500-M60
Source:
Monoclonal Mouse
Preparation:
Produced in BALB/c mice using highly pure recombinant Human IP-10 as the immunizing antigen. This antibody was purified from cell culture by Protein A affinity chromatography.
Immunogen:
E.coli derived Recombinant Human IP-10 (CXCL10) (PeproTech catalog #300-12)
Application:
ELISA
Sandwich
In a sandwich ELISA (assuming 100 μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human IP-10 when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Human IP-10 (500-P93Bt) as the detection antibody at a concentration of approximately 0.25 μg/ml. 

ELISA: Sandwich
?
Western Blot
To detect Human IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml (non-reducing) or at a concentration of 1.0-2.0 μg/ml (reducing). When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IP-10 is 0.20-0.40 ng/lane under non-reducing conditions and 2.00-4.00 ng/lane under reducing conditions. 

Western Transfer (Western Blot)
?
?
?
?
?
Research Interests:
Angiogenesis/ Cardiovascular
Chemotaxis
Immune System
Inflammation
Neurobiology
Proliferation
Wound Healing
Allergy
Transplantation
?
?
?
Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.