Size??100ug
Cat code??310003
Species Cross-reactivity??H,M,Mk,R
Key Application??WB
Host??Rabbit
Antibody type??Polyclonal antibody
Purified method??Affinity purified
Isotype??
Molecular Weight??88kDa
Sensitivity??The antibody detects endogenous level of total STAT3 protein.
Immunogen??Peptide sequence around aa. 725~729 (P-M-S-P-R) derived from Human STAT3.
UniProt Number??P40763
GeneBank ID??NM_003150.3
Subcellular location??Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
Formulation??Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Altername??1110034C02Rik ;Acute Phase Response Factor ;Acute-phase response factor ; APRF ;APRF ;AW109958 ;DNA binding protein APRF ;FLJ20882 ;HIES ;MGC128731 ;MGC16063 ;MGC93551 ;Signal transducer and activator of transcription 3 (acute-phase response factor) ;Signal transducer and activator of transcription 3 ;Signal Transductor and Activator of Transcription 3 ;Signal Transductor and Activator of Transcription 3 ;STAT 3 ;Stat3 ;STAT3_HUMAN ;Transcription factor .
Gene_symbol??APRF; HIES
Summary??The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
UniPort summary  Function??Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
Application Images :??
Western blot detection of STAT3 antibody in COS7, PC12, 3T3, Hela cell lysates using STAT3 antibody(dilution 1:300). Predicted band size:88KDa.Observed band size:88KDa.