Cdc42-GTP 小鼠單抗/Anti-Cdc42-GTP Monoclonal Antibody
產品名稱： Cdc42-GTP 小鼠單抗/Anti-Cdc42-GTP Monoclonal Antibody
英文名稱： Anti-Cdc42-GTP Monoclonal Antibody
產品編號： 26905
產品價格： null
產品產地： 美國
品牌商標： Neweast Biosciences
更新時間： null
使用范圍： IP, IHC, IF
- 聯系人 :
- 地址 : 武漢東湖新技術開發區高新大道666號武漢國家生物產業基地項目（生物創新園）B.C.D區研發樓B3-3棟3樓
- 郵編 :
- 所在區域 : 湖北
- 電話 : 181****2833 點擊查看
- 傳真 : 點擊查看
- 郵箱 : nebio0923@163.com
| 貨號: ??26905 |
| 規格: ??30 μL |
| 基因符號: ??CDC42 |
| 描述: ??Anti-Cdc42-GTP Monoclonal Antibody |
| 背景介紹: ??Small GTPases are a super-family of cellular signaling regulators. Cdc42 belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Cdc42, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). |
| 免疫原: ??Recombinant full length protein of active Cdc42 |
| 經過測試的應用: ??IP, IHC |
| 推薦稀釋比: ?? IP: 1 μg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| 濃度: ??1 mg/ml |
| 宿主: ??Mouse |
| 形式: ??Liquid |
| 克隆性: ??Monoclonal |
| 亞型: ??IgG1 |
| 純度: ??Purified from ascites |
| 防腐劑:???No |
| 成分:???PBS (without Mg2+?and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 種屬反應性: ??Anti-Cdc42-GTP monoclonal antibody recognizes active Cdc42 from vertebrates. |
| 儲存條件: ??Store at -20°C. Avoid repeated freezing and thawing |
IP-蛋白質印跡: ?? IP/WB analysis of active Cdc42 protein. Purified recombinant Cdc42 proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-Cdc42-GTP mouse monoclonal antibody (Cat. # 26905). After SDS/PAGE, the membrane filter was probed with anti-Cdc42 mouse monoclonal antibody (Cat. # 26008). |
免疫熒光: Immunofluorescent labeling of Cdc42-GTP in MS1 mouse endothelial cells. The MS1 cells cultured on glass coverslips (Fisher) in DMEM containing 5% FBS were fixed with 4% paraformaldehyde for 10 minutes at RT (room temperature) and permeabilized in PBSN (PBS+0.1%NP40) for 15 minutes at RT. The cells were stained with primary antibody (Cdc42-GTP) at 1: 25 dilution in Invitrogen’s CAS-BLOCK (00-8120) reagent at RT for 1 hour. After brief washing in PBSN (3x5min), a secondary Alexa555 conjugated goat anti mouse antibody (Invitrogen A21424) was applied in 1: 200 in CAS-BLOCK at RT for 1 hour. The coverslips were then washed in PBSN (3x5min) and mounted using vectorsheld’s hard set mounting medium (H-1500), and examined using a Zeiss inverted fluorescence microscope. |
