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• 特異性The immunising sequence is found in both beta III and beta IV tubulin. ab18207 detects human neuron specific beta III Tubulin protein specifically and cleanly but not as strongly as it detects the equivalent mouse and rat protein.
• 經測試應用IHC-FoFr,?Flow Cyt,?IHC - Wholemount,?IHC-P,?IHC-P,?IHC-Fr,?ICC/IF,?WBmore details
• 種屬反應性
  與反應:?Mouse, Rat, Human, Pig, Marmoset (common), Dogfish/Catshark
• 免疫原

  Synthetic peptide conjugated to KLH derived from within residues

  350 to the C-terminus of Human neuron specific beta III Tubulin.

  • 陽性對照
    • Human, Mouse, Rat or Dogfish/Catshark Brain Tissue ICC/IF: SKNSH, Neuro-2A and NGF-differentiated PC12 cells IHC: Hu Cerebellum (FFPE tissue) Flow Cytometry: U-87MG cells, Neuro 2A cells.

  性能

  • 形式Liquid
  • 存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存儲溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • 濃度Batch dependent within range:?100 μg 濃度為 0.5 - 1 mg/ml?
    

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  • 純度Immunogen affinity purified
  • 克隆多克隆
  • 同種型IgG
  • 研究領域
    • Neuroscience
    ?
    • Cell Adhesion Proteins
    ?
    • Cytoskeletal Proteins
    ?
    • Microtubules

    • Signal Transduction
    ?
    • Cytoskeleton / ECM
    ?
    • Cytoskeleton
    ?
    • Microtubules
    • Tubulin

    • Tags & Cell Markers
    ?
    • Cell Type Markers
    ?
    • Neuroscience Markers
    ?
    • Neuronal

  應用

  Our?Abpromise guarantee?covers the use of?ab18207?in the following tested applications.

  The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  應用	Abreviews	說明
  IHC-FoFr	?	Use at an assay dependent concentration. PubMed: 20568963
  Flow Cyt	?	Use 0.01μg for 106?cells. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
  IHC - Wholemount	?	Use at an assay dependent concentration. PubMed: 25383879
  IHC-P	?	1/2000.
  IHC-P	?	1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  IHC-Fr	?	Use at an assay dependent concentration.
  ICC/IF	?	Use a concentration of 1 - 5 μg/ml.
  WB	?	Use a concentration of 1 μg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 50 kDa).Can be blocked with?Human beta III Tubulin peptide (ab18660).

  靶標

  • 功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
  • 組織特異性Expression is primarily restricted to central and peripheral nervous system.
  • 疾病相關Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.
  • 序列相似性Belongs to the tubulin family.
  • 結構域The highly acidic C-terminal region may bind cations such as calcium.
  • 翻譯后修飾Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • 細胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 數據庫鏈接
    • Entrez Gene: 10381?Human
    • Entrez Gene: 22152?Mouse
    • Entrez Gene: 246118?Rat
    • Omim: 602661?Human
    • SwissProt: Q13509?Human
    • SwissProt: Q9ERD7?Mouse