Anti-beta III Tubulin抗體-抗體-抗體-生物在線

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深圳市宇德立生物科技有限公司
Anti-beta III Tubulin抗體

Anti-beta III Tubulin抗體

商家詢價(jià)

產(chǎn)品名稱： Anti-beta III Tubulin抗體

英文名稱： beta 3 tubulin antibody beta-4 antibody CDCBM antibody CFEOM3A antibody M(beta)3 antibody M(beta)6 antibody MC1R antibody Neuron specific beta III Tub

產(chǎn)品編號(hào)： ab18207

產(chǎn)品價(jià)格： null

產(chǎn)品產(chǎn)地： 英國(guó)

品牌商標(biāo)： abcam

更新時(shí)間： null

使用范圍：

深圳市宇德立生物科技有限公司
  • 聯(lián)系人 :
  • 地址 : 深圳市寶安區(qū)西鄉(xiāng)寶民二路賢基大廈4E
  • 郵編 :
  • 所在區(qū)域 : 廣東
  • 電話 : 133****4454 點(diǎn)擊查看
  • 傳真 : 點(diǎn)擊查看
  • 郵箱 : 1484332550@qq.com

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  • 特異性The immunising sequence is found in both beta III and beta IV tubulin. ab18207 detects human neuron specific beta III Tubulin protein specifically and cleanly but not as strongly as it detects the equivalent mouse and rat protein.
  • 經(jīng)測(cè)試應(yīng)用IHC-FoFr,?Flow Cyt,?IHC - Wholemount,?IHC-P,?IHC-P,?IHC-Fr,?ICC/IF,?WBmore details
  • 種屬反應(yīng)性
    與反應(yīng):?Mouse, Rat, Human, Pig, Marmoset (common), Dogfish/Catshark
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues

    350 to the C-terminus of Human neuron specific beta III Tubulin.

    • 陽(yáng)性對(duì)照
      • Human, Mouse, Rat or Dogfish/Catshark Brain Tissue ICC/IF: SKNSH, Neuro-2A and NGF-differentiated PC12 cells IHC: Hu Cerebellum (FFPE tissue) Flow Cytometry: U-87MG cells, Neuro 2A cells.

    性能

    應(yīng)用

    Our?Abpromise guarantee?covers the use of?ab18207?in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    應(yīng)用 Abreviews 說(shuō)明
    IHC-FoFr ? Use at an assay dependent concentration. PubMed: 20568963
    Flow Cyt ? Use 0.01μg for 106?cells.

    ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
    IHC - Wholemount ? Use at an assay dependent concentration. PubMed: 25383879
    IHC-P ? 1/2000.
    IHC-P ? 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    IHC-Fr ? Use at an assay dependent concentration.
    ICC/IF ? Use a concentration of 1 - 5 μg/ml.
    WB ? Use a concentration of 1 μg/ml. Detects a band of approximately 50-55 kDa (predicted molecular weight: 50 kDa).Can be blocked with?Human beta III Tubulin peptide (ab18660).

    靶標(biāo)

    • 功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
    • 組織特異性Expression is primarily restricted to central and peripheral nervous system.
    • 疾病相關(guān)Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.
    • 序列相似性Belongs to the tubulin family.
    • 結(jié)構(gòu)域The highly acidic C-terminal region may bind cations such as calcium.
    • 翻譯后修飾Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    • 細(xì)胞定位Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 數(shù)據(jù)庫(kù)鏈接