Broad cell line compatibility-kit includes siPORT Amine and siPORT NeoFX transfection agents.
 Faster protocol optimization-kit includes ready-to-transfect GAPDH and negative siRNA controls.
The choice of transfection agent is critical for gene silencing experiments. Every transfection agent has a different delivery efficiency when used with different cell types, and the overall transfection efficiency and degree of gene silencing depend on the nature of the transfection agent/siRNA complex. Whether you are new to RNAi research or you want to expand your experiments to include different cell lines, the Silencer siRNA Transfection II Kit enables you to optimize your transfection protocols for specific cell types. The Silencer siRNA Transfection II Kit includes siPORT Amine Transfection Agent (a polyamine mixture) and the new siPORT NeoFX Transfection Agent (a cationic and neutral lipid mixture). These reagents support efficient, reproducible siRNA transfections in different cell types. Furthermore, both can be used to transfect cells during subculturing-saving a full day of valuable time-without increased cytotoxicity. In addition to containing a choice of transfection agents, this kit includes a well-characterized GAPDH synthetic siRNA that can be used to optimize transfection conditions. Results can also be verified at the protein level with GAPDH-specific antibodies. A scrambled, negative control siRNA is also provided to confirm the specificity of the siRNA of interest and to demonstrate the absence of nonspecific, toxic effects.