TAXIScan-FL熒光細胞動態分析系統
產品名稱: TAXIScan-FL熒光細胞動態分析系統
英文名稱: Fluorescent cell mobility analysis device TAXIScan-FL
產品編號: TAXIScan-FL
產品價格: 0
產品產地: 日本
品牌商標: effectorcell
更新時間: null
使用范圍: null
- 聯系人 :
- 地址 : 北京市海淀區西三旗上奧世紀中心A座9層906
- 郵編 :
- 所在區域 : 北京
- 電話 : 186****1725 點擊查看
- 傳真 : 點擊查看
- 郵箱 : 787852745@qq.com
制造商(Manufacturer):日本ECI株式會社(ECI Inc., Japan )
主要技術指標(Main technical indicators):
物鏡:10×20×40×100×(Objective lens: 10×20×40×100×)
熒光濾塊:B/G/R (Fluorescent filter block: B/G/R)
樣品量:≤100個細胞(Sample amount: 100 or less cells)
溫度控制:室溫+ 3℃~40℃(Holder temperature control: room temperature+ 3℃~40℃)
硅基底芯片:通道深度4μm,5μm ,6μm,8μm(Chip terrace depth:4, 5, 6, or 8μm)
12個獨立通道,可同時進行12例試驗(12 channels, up to 12 concurrent assays)
自動聚焦系統(Autofocus system)
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制造商(Manufacturer):日本ECI株式會社(ECI Inc., Japan )
主要技術指標(Main technical indicators):
物鏡:10×20×40×100×(Objective lens: 10×20×40×100×)
熒光濾塊:B/G/R (Fluorescent filter block: B/G/R)
樣品量:≤100個細胞(Sample amount: 100 or less cells)
溫度控制:室溫+ 3℃~40℃(Holder temperature control: room temperature+ 3℃~40℃)
硅基底芯片:通道深度4μm,5μm ,6μm,8μm(Chip terrace depth:4, 5, 6, or 8μm)
12個獨立通道,可同時進行12例試驗(12 channels, up to 12 concurrent assays)
自動聚焦系統(Autofocus system)
動態影像實時記錄 (Data store as movie image file)
計算機分析系統,包含濃度梯度的精確測量,自動統計細胞數量,細胞形態變化、遷移速度、遷移方向等統計學分析。
主要功能(Main function):硅基底芯片,其上嵌刻的水平通道可形成化學趨化因子濃度梯度,用于測定濃度梯度依賴細胞的功能,如趨化,脫顆粒。水平通道的深度小于懸浮細胞的直徑,其內可觀測細胞形態學變化和增殖遷移過程。細胞趨化分析不僅包括中性粒細胞、嗜酸性粒細胞、單核細胞、淋巴細胞等外周血白細胞,也包括各種癌細胞和培養細胞,如平滑肌細胞、內皮細胞、神經細胞、干細胞等。還可分析蛋白質及細胞相互作用、細胞信號轉導、細胞骨架、鈣流入、活性氧代謝等??蓱糜谮吇蜃蛹八幬锖Y選、炎癥、過敏反應、腫瘤、神經、免疫、心血管、干細胞等方面的研究。
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日本ECI株式會社細胞動態可視化系統設備TAXIScan-FL,是全新光學動態成像與活體細胞處理技術的完美結合,本設備采用專利TAXIScan技術,具有獨立知識產權,其核心部件為硅基底芯片,其上嵌刻的水平通道可形成化學趨化因子濃度梯度;水平通道的深度精度小于懸浮細胞的直徑,可精確到微米級別,其內可觀測細胞形態學變化和增值遷移過程;成像部件冷光CCD相機定位于觀測平面以下,配有高性能透鏡和同軸反照明裝置;基于以上的革命性技術使實驗只需100個甚至更少的細胞樣本;根據實驗具體要求自定義設置實驗條件參數。
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Fluorescent filter block capacity |
Up to 5 blocks |
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Applicable fluorescent molecules |
Most commercial fluorphores |
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Bright field optical system |
Reflection plane image system |
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Objective lens |
10X 20X 40X and 100X |
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Focus system |
Autofocus compatible |
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Number of channels |
12 |
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Holder temperature control |
Room temperature + 3℃~40℃ |
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Light source |
Mercury lamp |
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Camera |
Monochrome cooled CCD camera |
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Equipment control and data analyzing software |
NIS-elements (manufactured by Nikon ) |
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Main body dimension |
Width 900mm X depth 750mm X height(excluding monitor)1100mm |
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Rated voltage |
AC 100V 50/60Hz |
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Wattage |
600VA |
TAXIScan is ECI's unique technology to form chemoattractant concentration gradient in a small space so as to observe cellular chemotaxis movement with good repeatability. Existing model of EZ-TAXIScan with a mere 10X objective lens, limited conventional bright-field observation and up to 6 sample simultaneous assays is appreciated well for its easy use of TAXIScan functions.
Newly introduced TAXIScan-FL makes fluorescent observation possible. The equipment is mounted with up to 40X objective lens and bright field observation system with the use of differential interferometry. The number of simultaneous assay samples is increased up to 12 so that higher analysis with enhanced throughput can be anticipated.

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[NEW]Chemotactic Cell Movies are now available |
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- The equipment enables fluorescent observation for various types of cell performing chemotaxis movement. Mounted with high-power objective lens (up to 40X)and differential interferometry optical system enable clear image acquisition at bright field observation. The employment of autofocus mechanism also enables stable focus for extended period to time.
- Simultaneous observation of transient increase (in fluorescent strength) of intracellular calcium concentration and chemotaxis movement were performed using cells stained with fluorescent calcium indicator (Fluo-3) (cell: human monocyte, chemoattractant: hMIP-1a)
- EZ-TAXIScan made observation of degranulation phenomena in mast cell possible. In contrast, TAXIScan-FL enables bright field observation in much higher magnification and differencial interferometry. By doing fluorescent straining for mast cells (blue : nucleus, green : calcium concentration, orange : degranulation), it becomes possible to observe transient calcium concentration increase in each cell before degranulation, etc. more in detail.
- Measurement record
- Main specification

