Active Gα13
Catalog Number: CR-M26902
Gene Symbol: Gna13
Description: Anti-Active Gα13-GTP Mouse Monoclonal Antibody

Background: Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored. Immunogen: Recombinant full length active Gα13 protein Tested applications: IP, IHC Recommended dilutions: 1 µg for 1~2 mg total cellular proteins Concentration: 1 mg/ml Host: mouse Clonality: Monoclonal Isotype: IgG1, IgG2b Purity : Purified from ascites Format: Liquid Storage buffer: Preservative: no Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity: Anti-active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates. Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles

Immunoprecipitation/Western blot:

IP/WB analysis of active Gα13 proteins. Purified Gα13 proteins were treated with GDP (lane 1) or GTPγS (lane 2). After immunoprecipitation with anti-active Gα13-GTP mouse monoclonal antibody (Cat. # CR-M26902), these proteins were separated by SDS/PAGE and western blotted with anti- Gα13 mouse monoclonal antibody (Cat. # CR-M26004).