Size??100ug
Cat code??200559
Species Cross-reactivity??H
Key Application??WB
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG2b
Molecular Weight??95kDa
Sensitivity??This antibody detects endogenous levels of EEF2 and does not cross-react with related proteins.
Immunogen??Purified recombinant human EEF2 protein fragments expressed in E.coli.
UniProt Number??P13639
GeneBank ID??NM_001961.3
Subcellular location??Cytoplasm
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername??eukaryotic translation elongation factor 2;EF2;EEF-2;EF-2;elongation factor 2;polypeptidyl-tRNA translocase
Gene_symbol??EF2; EF-2; EEF-2 
Summary??This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
UniPort summary  Function??Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Application Images :??
Western blot detection of EEF2 in HL-60 cell lysates using EEF2 antibody (1:1000 diluted).
Predicted band size:95KDa
Observed band size: 95KDa.