Anti-Active Gα i Mouse Monoclonal Antibody-抗體-抗體-生物在線
武漢紐斯特生物技術有限公司
Anti-Active Gα i Mouse Monoclonal Antibody

Anti-Active Gα i Mouse Monoclonal Antibody

商家詢價

產品名稱： Anti-Active Gα i Mouse Monoclonal Antibody

英文名稱： Anti-Active Gα i Mouse Monoclonal Antibody

產品編號： 26901

產品價格： null

產品產地： 美國

品牌商標： NewEastbio

更新時間： null

使用范圍： IP, IHC

武漢紐斯特生物技術有限公司
  • 聯系人 :
  • 地址 : 武漢市東湖新技術開發區光谷生物城B3-3棟2-3樓
  • 郵編 : 430074
  • 所在區域 : 湖北
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  • 郵箱 : neweast3@126.com

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Active Gαi-GTP [26901]
[30 μL]		 ?

Catalog Number:??26901
Gene Symbol:??Gnai
Description: ??Anti-Active Gα i Mouse Monoclonal Antibody
Background: ??Heterotrimeric G proteins are critical cellular signal transducers. Gα i represents one sub-family of G proteins that could mediate the inhibition of adenylyl cyclases. Other biochemical and physiological functions of Gα i proteins are being explored.
Immunogen: ??Recombinant full length protein of active Gα i1
Applications: ??IP, IHC
Recommended Dilutions: ??1 μg for 1~2 mg total cellular proteins for IP;
???????????????????????????????????????????????????1:100 dilution for IHC
Concentration: ??1 mg/ml
Host: ??mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??IgG1
Purity: ??Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Anti-active Gα i antibody recognizes active Gα i1, Gα i2, and Gα i3 of vertebrates.
Storage Conditions:??Store at -20°C. Avoid freeze / thaw cycles
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Immunoprecipitation/Western blot:??
Gαi activation assay.
A. CHO cells were transfected with wild-type Gα i1 (lanes 1 and 2) or constitutively active Gα i1-Q204L (lane 3). Cell lysates were treated with GDP(lane 1) or GTPγS (lane 3). Lysates were then incubated with an anti-active Gα i monoclonal antibody (Cat. No.26901) (top panel). The precipitated active Gα i was immunoblotted with an anti-Gα i monoclonal antibody (Cat. No. 26003). The bottom panel shows the Western blot with anti-Gα i monoclonal antibody (Cat.No. 26003) of the cell lysates.
B. HEK293 cells stably expressing human m2 mAChR were treated with (lane 2) or without (lane 1)carbachol. Cell lysates were then incubated with an anti-active Gα i monoclonal antibody (Cat. No. 26901) (top panel).The precipitated active Gα i was immunoblotted with an anti-Gα i rabbit polyclonal antibody (Cat. No. 21006). The bottom panel shows the Western blot with anti-tubulin of the cell lysates.
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Publications:
1.??Identification of novel signalling roles and targets for Galpha and Gbetagamma downstream of the insulin-like growth factor 1 receptor in vascular smooth muscle cells
????Biochem J. 2013 Feb 15;450(1):209-19
2.??CRH activation of different signaling pathways results in differential calcium signaling in human pregnant myometrium before and during labor
????J Clin Endocrinol Metab. 2012 Oct;97(10):E1851-61
3.??CXCR4 activation defines a new subgroup of Sonic hedgehog-driven medulloblastoma
????Cancer Res. 2012 Jan 1;72(1):122-32
4.??Regulation of estradiol and progesterone production by CRH-R1 and -R2 is through divergent signaling pathways in cultured human placental trophoblasts
????Endocrinology. 2012 Oct;153(10):4918-28
5.??WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins
????Cell Signal. 2011 Mar;23(3):550-4
6.??Heterotrimeric Galpha(i) proteins are regulated by lipopolysaccharide and are anti-inflammatory in endotoxemia and polymicrobial sepsis
????Biochim Biophys Acta. 2011 Mar;1813(3):466-72
7.??Estrogen- and xenoestrogen-induced ERK signaling in pituitary tumor cells involves estrogen receptor-α interactions with G protein-αi and caveolin I
????Steroids Volume 77, Issue 5, April 2012, Pages 424–432
8.??Na/H exchanger regulatory factors control parathyroid hormone receptor signaling by facilitating differential activation of G(alpha) protein subunits
????J Biol Chem. 2010 Aug 27;285(35):26976-86
9.??Beta-arrestin- but not G protein-mediated signaling by the "decoy" receptor CXCR7
????Proc Natl Acad Sci U S A. 2010 Jan 12;107(2):628-32
10.??Structural basis for activation of trimeric Gi proteins by multiple growth factor receptors via GIV/Girdin
????Mol Biol Cell. 2014 Nov 5;25(22):3654-71
11.??Canonical and Non-canonical G-protein Signaling Helps Coordinate Actin Dynamics to Promote Macrophage Phagocytosis of Zymosan
????Mol Cell Biol. 2014 Nov 15;34(22):4186-99
12.??Fatty Acid-binding Protein 5 (FABP5) Regulates Cognitive Function Both by Decreasing Anandamide Levels and by Activating the Nuclear Receptor Peroxisome Proliferator-activated Receptor β/δ (PPARβ/δ) in the Brain
J Biol Chem. 2014 May 2;289(18):12748-58