Size??100ug
Cat code??200363
Species Cross-reactivity??E.coli
Key Application??WB-re
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG2b
Molecular Weight??24kDa
Sensitivity??This antibody detects endogenous levels DsbA and does not cross-react with related proteins.
Immunogen??Purified recombinant human DsbA protein fragments expressed in E.coli.
UniProt Number??P0AEG4
GeneBank ID??YP_003001429
Subcellular location??Periplasm
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername??null
Gene_symbol??null
Summary??Disulphide oxidoreductase (DsbA) is the major oxidase responsible for generation of disulfide bonds in proteins of E. coli envelope. It is a member of the thioredoxin superfamily. DsbA introduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA is reoxidized by dsbB. It is required for pilus biogenesis.
UniPort summary  Function??Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. It is required for pilus biogenesis
Application Images :??
Western blot detection of DsbA with Dsba recombinant protein using DsbA antibody (1:1000 diluted).
Predicted band size: 24KDa
Observed band size: 24KDa.
Western blot detection of DSBA in AKT1,AKT2,AKT3 and DSBA recombinant antigen fragments using DSBA antibody (1:1000 diluted).