羅氏地高&辛DIG標記檢測試劑盒KitII(化學發光)(Roche:11585614910)
產品名稱： 羅氏地高&辛DIG標記檢測試劑盒KitII(化學發光)(Roche:11585614910)
英文名稱： DIG High Prime DNA Labeling and Detection Starter Kit II：Kit for chemiluminescent detection with CSPD, ready-to-use
產品編號： 11585614910
產品價格： 0
產品產地： Roche
品牌商標： Roche
更新時間： null
使用范圍： null
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DIG高效DNA標記及檢測Starter Kit II說明書
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DIG高效DNA標記及檢測Starter Kit II
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DIG High Prime DNA Labeling and Detection Starter Kit II：Kit for chemiluminescent detection with CSPD, ready-to-use
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對于需要使用化學發光法檢測的用戶，我公司現提供了此新的DIG標記及檢測試劑盒。
與上述的StarterKit I不同，此新的StarterKit II所含的底物為CSPD，但它同樣有以下特點：
●能對10×10cm印跡膜作12個標記反應及24個檢測反應，所以特別適合初用者及需處理較少量樣品的用戶使用。
●包含DIG高效標記試劑，令標記效率更高，操作更方便。 ●包括DIG定量/對照試紙條及NBT/BCIP濃縮液，令您更易估計探針濃度，從而取得理想的信噪比。 ?
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產品號
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產品規格
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廠商
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1585614
11585614910
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Kit：1 kit (12 labeling reactions and 24 detection reactions)
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Roche
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DIG-High Prime DNA Labeling and Detection Starter Kit II
Application
DIG-High Prime labeled probes can be used in northern, Southern-, and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
Benefits
The High Prime method of labeling offers many benefits: in addition to the optimized labeling efficiency, plasmid, cosmid, and phage DNA, linearized or supercoiled can be used as templates, yielding the same labeling efficiency.
Product Description
Sensitivity and specificity:?Standard assay uses 1 μg of linearized pBR328. It is usual to expect 0.8 μg labeled DNA after 1 h of labeling, and 2.3 μg labeled DNA after 20 h reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot, loading 1 μg of digested placenta DNA.
Contents
1.?????? DIG-High Prime, 5x conc., 50 μl
2.?????? DIG-labeled Control DNA, 20 μl, (5 μg/ml) pBR328 (linearized with?Bam?HI)
3.?????? DNA Dilution Buffer, 3 x 1 ml
4.?????? Anti-digoxigenin-AP Conjugate, 50 μl
5.?????? CSPD, ready-to-use, 50 ml
6.?????? Blocking Solution, 10x conc., 4 x 100 ml
7.?????? DIG Easy Hyb Granules, 4 x 100 ml
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