Annexin V-EGFP/PI細胞凋亡檢測試劑盒是用EGFP標記了的Annexin V作為探針，來檢測細胞早期凋亡的發生。

其檢測原理為：在正常的活細胞中，磷脂酰絲氨酸（phosphotidylserine，PS）位于細胞膜的內側，但在早期凋亡的細胞中，PS 從細胞膜的內側翻轉到細胞膜的表面，暴露在細胞外環境中。Annexin-Ⅴ（膜聯蛋白-V）是一種分子量為35-36 kDa的Ca²⁺ 依賴性磷脂結合蛋白，能與PS高親和力結合。可通過細胞外側暴露的磷脂酰絲氨酸與凋亡早期細胞的胞膜結合。

另外，本試劑盒中還提供了碘化丙啶（Propidium Iodide，PI）用來區分存活的早期細胞和壞死或晚期凋亡細胞。PI是一種核酸染料，它不能透過正常細胞或早期凋亡細胞的完整的細胞膜，但可以透過凋亡晚期和壞死細胞的細胞膜而使細胞核染紅。因此，將Annexin V與PI聯合使用時，PI則被排除在活細胞（Annexin V^-/PI^-）和早期凋亡細胞（Annexin V⁺/PI^-）之外，而晚期凋亡細胞和壞死細胞同時被EGFP和PI結合染色呈現雙陽性（Annexin V⁺/PI⁺）。

本試劑盒適合使用流式細胞儀和熒光顯微鏡進行檢測。

檢測便捷、準確

冰袋（wet ice）運輸。-20 ℃避光保存，避免反復凍融，二年有效。

【注】：如果需要在短時間內多次重復使用，可以在4 ℃避光保存，半年有效。

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