SDS PAGE of native, human Grp78 protein.

GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5 nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia (Barnes), to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).

1. Cho, S. et al (2007). Mol Cell Biol 27(1): 368-83.
2. Yang, Y. et al. (1998) J Biol Chem 273:25552-25555.
3. Luo, S. et al (2006) 26 (15): 5688-97.
4. Yu, Z. et al. (1999) Exp Neurol. 15: 302-314.
5. Koomagi, R. et al. (1999) Anticancer Res. 19: 4333-4336.
6. Laquerre, S. et al. (1998) J. Virology 72: 4940-4949.
7. Dong, D. et al. (2005) Cancer Res 65(13): 5785-91.

發表文獻
1. Maddalo, D. et al. (2012). A Peptidic Unconjugated GRP78/BiP Ligand Modulates the Unfolded Protein Response and Induces Prostate Cancer Cell Death. PLoS ONE. 7(10): e45690. doi:10.1371/journal.pone.0045690

2. Ding, Y. et al. (2013). IL-17RA Is Essential for Optimal Localization of Follicular Th Cells in the Germinal Center Light Zone To Promote Autoantibody-Producing B Cells. Journal of Immunology. Published online. doi: 10.4049/jimmunol.1300479

3. Bruchmann, A. et al. (2013). Bcl-2 associated athanogene 5 (Bag5) is overexpressed in prostate cancer and inhibits ER-stress induced apoptosis. BMC Cancer. 13:96. doi:10.1186/1471-2407-13-96