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• 詳細(xì)信息：

• 美國BIOLOG公司獨(dú)家研發(fā)的產(chǎn)品 ECO板，特別為微生物群落分析和生態(tài)研究設(shè)計，Eco板上包含國際土壤微生物學(xué)家精選的用于微生物群落分析最常用的31種碳源，是國際上微生物生態(tài)功能多樣性研究的最典型和最權(quán)威方法之一。

  每塊96孔ECO板上有3組平行，每組31種碳源，3個陰性對照，加入同一樣品后，可以獲得3組平行數(shù)據(jù)。微生物群落對這31種碳源的特征性利用稱作該微生物群落的代謝指紋圖譜。從單個的微平板上的指紋圖譜就可以獲得大量的代謝信息。可用于純種及混合菌群(如土壤樣品)微生物功能多樣性研究。

  31種碳源可分為六大類，具體如下：

  單糖\糖苷\聚合糖類

  B2， D-木糖

  H1, a-D-乳糖

  A2, ?-甲基D-葡萄糖苷

  G2,葡萄糖-1-磷酸鹽

  E1, a-環(huán)狀糊精

  F1, 肝糖

  G1, D-纖維二糖

  氨基酸類，

  A4，L-精氨酸，

  B4，L-天冬酰胺酸

  C4 ? ?L-苯基丙氨酸

  D4, ? L-絲氨酸

  E4，L-蘇氨酸

  F4，甘氨酰-L-谷氨酸

  酯類

  B1, 丙酮酸甲酯

  C1, 吐溫40

  D1, 吐溫80

  A3, D-半乳糖酸γ內(nèi)酯

  醇類

  C2, I-赤藻糖醇

  D2, D-甘露醇

  H2, D,L-a-甘油

  胺類

  G4, 苯乙基胺

  H4, 腐胺

  E2, N-乙?；?D-葡萄胺

  酸類

  B3,D-半乳糖醛酸

  F2 D-氨基葡萄糖酸

  C3, 2-羥苯甲酸

  D3, 4-羥基苯甲酸

  E3,r-羥基丁酸

  F3, 衣康酸

  G3,a-丁酮酸

  H3, D-蘋果酸

  樣品簡單處理后(過濾或離心)，取上清液加到ECO板上培養(yǎng)多至5天時間，每隔一定時間用酶標(biāo)儀在590nm和750nm下測定OD值，OD值的大小反應(yīng)樣品微生物利用某一碳源底物的能力，將OD值導(dǎo)出后進(jìn)行分析，如AWCD值(平均吸光度)、豐富度、單孔的動力學(xué)分析、不同類碳源的比較等，并可用聚類軟件對不同樣品進(jìn)行聚類分析。

  值得一提的是ECO板不僅測定可培養(yǎng)微生物，還可測定部分不可培養(yǎng)微生物，只要微生物能利用碳源進(jìn)行代謝(并不增殖)，ECO板上顯色體系均可檢測出來。

  最常見的應(yīng)用是利用ECO板分析環(huán)境因素、根系、葉面、肥料、農(nóng)藥對土壤微生物菌群的生態(tài)功能多樣性的影響，比較不同樣品間或同一樣品不同時間的功能多樣性差異，比較環(huán)境修復(fù)前后微生物群落的功能多樣性差異，等等。

  BIOLOG鑒定板與培養(yǎng)基 Microplate Panels and Media GEN II & GEN III MicroPlate Consumables ? ·???????? Sold in increments of 10 plates/box ·???????? Consumable products are NON-RETURNABLE Catalog Number Description 1030 GEN III MicroPlate (for both Gram-negative & Gram-positive bacteria) 1011 GN2 MicroPlate (GEN II for Gram-negative bacteria) 1014 GP2 MicroPlate (GEN II for Gram-positive bacteria) 1007 AN MicroPlate (GEN II for Anaerobic bacteria) 1005 YT MicroPlate (GEN II for Yeasts) 1006 FF MicroPlate (GEN II for Filamentous Fungi) Special Purpose MicroPlates ·???????? Sold in increments of 10 plates/box ·???????? Consumable products are NON-RETURNABLE Catalog Number Description 1013 MT2 MicroPlate (for metabolic studies) 1511 SF-N2 MicroPlate (for metabolic characterization of spore-forming and conidia-forming gram-negative microbes) 1514 SF-P2 MicroPlate (for metabolic characterization of spore-forming and conidia-forming gram-positive microbes) 1506 ECO MicroPlate - 3 sets of the same 31 carbon sources in a 96 well Biolog MicroPlate. Used for characterization / community analysis) Biolog Prepared Plated Media ·???????? Sold in increments of 15 plates/pack Catalog Number Description 71102 BUG (Biolog Universal Growth) Agar w/ 5% sheep blood 71103 BUG (Biolog Universal Growth) Agar w/ 0.25% maltose1 71005 BUY (Biolog Universal Yeast) Agar1 Other Prepared Plated Media ·???????? Sold in increments of 10 plates/pack Catalog Number Description Bio-M1040 Tryptic Soy Agar (TSA) Bio-M1006 Tryptic Soy Agar (TSA) w/ 5% sheep blood Bio-M1012 Chocolate Agar, enriched 71007 BUA (Biolog Universal Anaerobe) Agar w/5% sheep blood Bio-M1065 R2A Agar 71106 2% Malt Extract Agar Biolog Dehydrated Media Catalog Number Description 70101 BUG (Biolog Universal Growth) Agar - 500 g jar (approx. 435 plates) 70007 BUA (Biolog Universal Anaerobe) Agar 70005 BUY (Biolog Universal Yeast) Agar - 500 g jar (approx. 415 plates) GEN III Starter Kits ·???????? Kits containing the consumables a new user would need to begin using the OmniLog System for bacterial indentifications Catalog Number Description 39993A-O 20 GEN III MicroPlates ·???????? 1 box of pipette tips (Ovation) ·???????? 1 CASE OF reservoirs ·???????? 1 package OF Inoculatorz (100 units individually packaged) ·???????? 20 tubes OF IF-A ·???????? 20 tubes OF IF-B 39993A-M 20 GEN III MicroPlates ·???????? 1 box of pipette tips (Matrix) ·???????? 1 CASE OF reservoirs ·???????? 1 package OF Inoculatorz (100 units individually packaged) ·???????? 20 tubes OF IF-A ·???????? 20 tubes OF IF-B 39993B 30 BUG+blood plated media for GEN III

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