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該單克隆抗體是采用合成的與人源MEK1蛋白Ala220周圍相一致的肽段免疫動(dòng)物而產(chǎn)生的。此抗體通過胺基端和辣根過氧化物酶的碳水化合物端鏈接。此HRP偶聯(lián)的抗體預(yù)期和未偶聯(lián)的MEK1/2 (D1A5) Rabbit mAb #8727顯示同樣的物種交叉反應(yīng)性。

Western Blotting

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Western blot analysis of extracts from NIH/3T3 and HeLa cells using MEK1/2 (D1A5) Rabbit mAb (HRP Conjugate).

使用MEK1/2 (D1A5) Rabbit mAb兔單抗 (HRP Conjugate)對(duì)NIH/3T3和HeLa細(xì)胞提取物進(jìn)行western blot分析。

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Background

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MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation (1-3). Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx (1-4). Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells (4). MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.

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MEK1、MEK2,也被稱為MAPK或Erk激酶，它們是雙特異性蛋白激酶，在絲裂原活化蛋白激酶級(jí)聯(lián)反應(yīng)中發(fā)揮作用，控制細(xì)胞生長(zhǎng)和分化(1-3)。MEK1、MEK2的活化是通過Raf樣分子對(duì)兩個(gè)絲氨酸（217 /221位點(diǎn)）的磷酸化而實(shí)現(xiàn)的，這兩個(gè)絲氨酸位點(diǎn)位于亞結(jié)構(gòu)域VIII的活化環(huán)內(nèi)。多種生長(zhǎng)因子和細(xì)胞因子以及膜去極化和鈣內(nèi)流都能激活MEK1/2(1-4)。組成型活化的MEK1/2能夠誘導(dǎo)NIH/3T3細(xì)胞的轉(zhuǎn)化和PC-12細(xì)胞的分化(4)。MEK蛋白通過將p44、p42 MAPK激酶上位于亞結(jié)構(gòu)域VIII的活化環(huán)內(nèi)的絲氨酸和蘇氨酸磷酸化，激活p44、 p42 MAPK激酶。

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