Product: Anti-CD56 (PE-labeled), clone C5.9
Cat. No: MC-863 (100 tests)
Background:
CD56, M.W. 150,000, is expressed on
approximately 10-25% of human peripheral
blood lymphocytes. CD56 (NKH-1) is expressed
on human peripheral blood natural killer cells,
representing a pan NK-cell antigen. Expressed
on non-MHC-restricted cytotoxic T cells.
Specificity:
Reacts with human CD56.
Species Reactivity:
Human, others not tested.
Ig Isotype:
Mouse IgG2b kappa
Format:
PE labeled monoclonal antibody supplied in 1 ml
PBS, 0.08% sodium azide, and 0.2% protein
carrier.
Storage and Stability:
Store at 4°C. Do not freeze.
Applications and Suggested Dilutions:

Immunofluorescence

Flow cytometry:
USE:
PBMC: Add 10 μl of mAb/106 PBMC in 100 μl
PBS. Mix gently and incubate for 15 minutes at
2 to 8°C. Wash twice with PBS and analyze or
fix with 0.5% v/v of paraformaldehyde in PBS
and analyze.
WHOLE BLOOD: Add10 μl of mAb/100 μl of
whole blood. Mix gently and incubate for 15
minutes at room temperature (20°C). Lyse the
whole blood. Wash once with PBS and analyze
or fix with 0.5% v/v of paraformaldehyde in PBS
and analyze. See instrument manufacturer's
instructions for lysed whole blood and
immunofluorescence analysis with a flow
cytometer or microscope.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product