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?英文名稱PCNA?(Nuclear?Loading?Control)
中文名稱增殖細胞核抗原（核內參）重組兔單克隆抗體
別????名Cyclin;?DNA?polymerase?delta?auxiliary?protein;?HGCN8729;?MGC8367;?Mutagen-sensitive?209?protein;?Pcnaclin;?PCNAR;?Polymerase?delta?accessory?protein;?Proliferating?Cell?Nuclear?Antigen;?PCNA_HUMAN.??
產品類型內參抗體?
研究領域腫瘤??細胞生物??染色質和核信號??細胞周期蛋白??
抗體來源Rabbit
克隆類型Monoclonal
克?隆?號1G3
交叉反應Human,?Mouse,?Rat,?
產品應用WB=1:1000-5000?IP=1:10-50?IHC-P=1:100-500?IHC-F=1:100-500?ICC=1:50-200?IF=1:100-500?（石蠟切片需做抗原修復）
not?yet?tested?in?other?applications.
optimal?dilutions/concentrations?should?be?determined?by?the?end?user.
分?子?量29kDa
細胞定位細胞核?
性????狀Liquid
濃????度1mg/ml
免?疫?原Recombinant?human?PCNA?protein:?
亞????型IgG
純化方法affinity?purified?by?Protein?A
儲?存?液0.01M?TBS(pH7.4)?with?1%?BSA,?0.03%?Proclin300?and?50%?Glycerol.
保存條件Shipped?at?4℃.?Store?at?-20?°C?for?one?year.?Avoid?repeated?freeze/thaw?cycles.
PubMedPubMed
產品介紹Proliferating?cell?nuclear?antigen?(PCNA)?is?a?28kDa?nuclear?protein?associated?with?the?cell?cycle,?a?nuclear?protein?vital?for?cellular?DNA?synthesis.?Proliferating?cell?nuclear?antigen?was?originally?identified?by?immunofluorescence?as?a?nuclear?protein?whose?appearance?correlated?with?the?proliferate?state?of?the?cell.?PCNA?is?required?for?replication?of?DNA?in?vitro?and?has?been?identified?as?the?auxiliary?protein?(cofactor)?for?DNA?polymerase?delta.?The?anti-PCNA?antibodies?react?with?the?nuclei?of?proliferating?cells.?PCNA?is?essential?for?cellular?DNA?synthesis?and?is?also?required?for?the?in?vitro?replication?of?simian?virus?40?(SV40)?DNA?where?it?acts?to?coordinate?leading?and?lagging?strand?synthesis?at?the?replication?fork.?The?PCNA?protein?may?fulfil?several?separate?roles?in?the?cell?nucleus?associated?with?changes?in?its?antigenic?structure.

Function:
Auxiliary?protein?of?DNA?polymerase?delta?and?is?involved?in?the?control?of?eukaryotic?DNA?replication?by?increasing?the?polymerase's?processibility?during?elongation?of?the?leading?strand.?Induces?a?robust?stimulatory?effect?on?the?3'-5'?exonuclease?and?3'-phosphodiesterase,?but?not?apurinic-apyrimidinic?(AP)?endonuclease,?APEX2?activities.?Has?to?be?loaded?onto?DNA?in?order?to?be?able?to?stimulate?APEX2.?Plays?a?key?role?in?DNA?damage?response?(DDR)?by?being?conveniently?positioned?at?the?replication?fork?to?coordinate?DNA?replication?with?DNA?repair?and?DNA?damage?tolerance?pathways.?Acts?as?a?loading?platform?to?recruit?DDR?proteins?that?allow?completion?of?DNA?replication?after?DNA?damage?and?promote?postreplication?repair:?Monoubiquitinated?PCNA?leads?to?recruitment?of?translesion?(TLS)?polymerases,?while?'Lys-63'-linked?polyubiquitination?of?PCNA?is?involved?in?error-free?pathway?and?employs?recombination?mechanisms?to?synthesize?across?the?lesion.

Subunit:
Homotrimer.?Forms?a?complex?with?activator?1?heteropentamer?in?the?presence?of?ATP.?Interacts?with?EXO1,?POLH,?POLK,?DNMT1,?ERCC5,?FEN1,?CDC6?and?POLDIP2.?Interacts?with?APEX2;?this?interaction?is?triggered?by?reactive?oxygen?species?and?increased?by?misincorporation?of?uracil?in?nuclear?DNA.?Forms?a?ternary?complex?with?DNTTIP2?and?core?histone.?Interacts?with?KCTD10?and?PPP1R15A?(By?similarity).?Interacts?with?POLD1,?POLD3?and?POLD4.?Interacts?with?BAZ1B;?the?interaction?is?direct.?Interacts?with?HLTF?and?SHPRH.?Interacts?with?NUDT15.?Interaction?is?disrupted?in?response?to?UV?irradiation?and?acetylation.?Interacts?with?CDKN1A/p21(CIP1)?and?CDT1;?interacts?via?their?PIP-box?which?also?recruits?the?DCX(DTL)?complex.?Interacts?with?DDX11.?Interacts?with?EGFR;?positively?regulates?PCNA.?Interacts?with?PARPBP.?Interacts?(when?ubiquitinated)?with?SPRTN;?leading?to?enhance?RAD18-mediated?PCNA?ubiquitination.?Interacts?(when?polyubiquitinated)?with?ZRANB3.?Interacts?with?SMARCAD1.?Interacts?with?CDKN1C.?Interacts?with?KIAA0101/PAF15?(via?PIP-box).

Subcellular?Location:
Nucleus.?Note=Forms?nuclear?foci?representing?sites?of?ongoing?DNA?replication?and?vary?in?morphology?and?number?during?S?phase.?Together?with?APEX2,?is?redistributed?in?discrete?nuclear?foci?in?presence?of?oxidative?DNA?damaging?agents.

Post-translational?modifications:
Following?DNA?damage,?can?be?either?monoubiquitinated?to?stimulate?direct?bypass?of?DNA?lesions?by?specialized?DNA?polymerases?or?polyubiquitinated?to?promote?recombination-dependent?DNA?synthesis?across?DNA?lesions?by?template?switching?mechanisms.?Following?induction?of?replication?stress,?monoubiquitinated?by?the?UBE2B-RAD18?complex?on?Lys-164,?leading?to?recruit?translesion?(TLS)?polymerases,?which?are?able?to?synthesize?across?DNA?lesions?in?a?potentially?error-prone?manner.?An?error-free?pathway?also?exists?and?requires?non-canonical?polyubiquitination?on?Lys-164?through?'Lys-63'?linkage?of?ubiquitin?moieties?by?the?E2?complex?UBE2N-UBE2V2?and?the?E3?ligases,?HLTF,?RNF8?and?SHPRH.?This?error-free?pathway,?also?known?as?template?switching,?employs?recombination?mechanisms?to?synthesize?across?the?lesion,?using?as?a?template?the?undamaged,?newly?synthesized?strand?of?the?sister?chromatid.?Monoubiquitination?at?Lys-164?also?takes?place?in?undamaged?proliferating?cells,?and?is?mediated?by?the?DCX(DTL)?complex,?leading?to?enhance?PCNA-dependent?translesion?DNA?synthesis.?Sumoylated?during?S?phase.
Acetylated?in?response?to?UV?irradiation.?Acetylation?disrupts?interaction?with?NUDT15?and?promotes?degradation.
Phosphorylated.?Phosphorylation?at?Tyr-211?by?EGFR?stabilizes?chromatin-associated?PCNA.

Similarity:
Belongs?to?the?PCNA?family.

SWISS:
P12004

Gene?ID:
5111

Database?links:
Entrez?Gene:?515499?Cow

Entrez?Gene:?5111?Human

Entrez?Gene:?18538?Mouse

Entrez?Gene:?25737?Rat

Omim:?176740?Human

SwissProt:?Q3ZBW4?Cow

SwissProt:?P12004?Human

SwissProt:?P17918?Mouse

SwissProt:?P04961?Rat

Unigene:?147433?Human

Unigene:?728886?Human

Unigene:?7141?Mouse

Unigene:?223?Rat



Important?Note:
This?product?as?supplied?is?intended?for?research?use?only,?not?for?use?in?human,?therapeutic?or?diagnostic?applications.

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產品圖片
Sample:
Lane 1: Testis (Rat) Lysate at 40 ug
Lane 2: Testis (Mouse) Lysate at 40 ug
Lane 3: SiHa (Human) Cell Lysate at 30 ug
Lane 4: MCF-7 (Human) Cell Lysate at 30 ug
Lane 5: A549 (Human) Cell Lysate at 30 ug
Lane 6: U937 (Human) Cell Lysate at 30 ug
Lane 7: NIH/3T3(Mouse) Cell Lysate at 30 ug
Lane 8: HepG2 (Human) Cell Lysate at 30 ug
Lane 9: 293T (Human) Cell Lysate at 30 ug
Lane 10: A431 (Human) Cell Lysate at 30 ug
Primary: Anti-PCNA (bsm-52347R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32’36 kD
Observed band size: 32 kD
Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCNA (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-52347R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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