Size??100ug
Cat code??200145
Species Cross-reactivity??M
Key Application??WB,FC,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG1
Molecular Weight??35kDa
Sensitivity??This antibody detects endogenous levels of Sox2 and does not cross-react with related proteins.
Immunogen??Purified recombinant mouse Sox2 protein fragments expressed in E.coli
UniProt Number??P48431
GeneBank ID??NM_011443
Subcellular location??Nucleus.
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername??ANOP3;cb236;Delta EF2a;lcc;MCOPS3;MGC148683;MGC2413;RGD1565646;Sex determining region Y box 2;SOX 2;Sox2;SOX2_HUMAN;SRY (sex determining region Y) box 2;SRY box containing gene 2;SRY related HMG box 2;SRY related HMG box gene 2;SRY-box 2;Transcription factor SOX 2;Transcription factor SOX-2;ysb.
Gene_symbol??lcc;ysb;Sox-2.
Summary??
UniPort summary  Function??Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1,FGF4,UTF1 and ZFP206 by similarity.Critical for early embryogenesis and for embryonic stem cell pluripotency.May function as a switch in neuronal development.Downstream SRRT target that mediates the promotion of neural stem cell self-renewal by similarity.Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation by similarity.
Application Images :??
Western blot detection of Sox2 antibody in F9 cell lysates using Sox2 antibody (1:1000 diluted).Predicted band size:35KDa.Observed band size:35KDa.
Immunocytochemistry of COS7 cells using anti-Sox2 antibody diluted 1:150.
Flow Cytometry analysis of F9 cells stained with Sox2 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
Western blot detection of Sox2 in mES cell lysates using Sox2 antibody(1:1000 diluted).Predicted band size:35KDa,Observed band size:35KDa.Kindly provided by Dr. Qintong Li at the College of Life Sciences, Sichuan University