Recombinant Human ALPL Protein,His Tag
產品名稱： Recombinant Human ALPL Protein,His Tag
英文名稱： Recombinant Human ALPL Protein,His Tag
產品編號： 93059ES
產品價格： 詢價
產品產地： Yeasen
品牌商標： Yeasen
更新時間： 2025-08-20T17:16:09
使用范圍： null
翌圣生物科技（上海）股份有限公司
- 聯系人 : 李自轉
- 地址 : 上海市浦東新區天雄路166弄一號樓三層南單元
- 郵編 : 200030
- 所在區域 : 上海
- 電話 : 139****5640 點擊查看
- 傳真 : 點擊查看
- 郵箱 : lizizhuan@yeasen.com
- 二維碼 : 點擊查看
產品介紹
Tissue non-specific alkaline phosphatase (TNAP) is an isoenzyme of alkaline phosphatase encoded by the ALPL gene in the human body. TNAP is mainly present in tissues such as bone, liver, and kidneys. It plays a crucial role in bone by participating in the metabolism of phosphates and the mineralization process of the skeleton. Additionally, TNAP also plays a role in fetal development and growth. In clinical practice, the activity level of TNAP is also used as a diagnostic marker for bone diseases and liver diseases. Research also indicates that TNAP may play an important role in human diseases, including bone disorders such as osteoporosis and rickets, as well as other conditions such as cancer and inflammatory diseases. Tissue non-specific alkaline phosphatase (TNAP) is found in many different organs, but abundant in the skeletal, hepatic and renal tissues. TNAP is a homodimeric protein and in its structure, in addition to one phosphate anion, each monomer is composed of three metallic ions (one Mg2+ and two Zn2+ cations). Each subunit contains an extended central core β-beach with α-helices, while an extended N-terminal α-helix has a “crown domain”. The crown domain can be characterised as a loose interfacial loop with amino acid residues involved in stabilizing the binding of non-competitive inhibitors to the enzyme.
產品性質
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產品別稱
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AP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP
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種屬
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Human
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表達序列
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Leu18-Ser502
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Accession
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P05186-1
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標簽
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C-His Tag
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表達系統
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HEK293
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分子量
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The protein has a predicted MW of 55.3 kDa
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純度
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> 95% as determined by SDS-PAGE
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內毒素
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< 1.0 EU per μg by the LAL method.
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制劑
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Supplied as a 0.2 μm filtered solution of PBS, pH7.4.
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外觀
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Liquid
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生物活性
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ELISA: Human ALPL Protein; His Tag was immobilized at 1 μg/ml (100 μL/well). Increasing concentrations of Anti-His mIgG2a Antibody were added.
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產品特色
高純度、高活性、低內毒素、高批間一致性
應用案例
SDS-PAGE
Figure 1.On SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Figure 2.Human ALPL Protein; His Tag was immobilized at 1 μg/ml (100 μL/well). Increasing concentrations of Anti-His mIgG2a Antibody were added.
存儲條件
-85 ~ -65℃保存，收到貨之日起，6個月有效期。避免反復凍融，建議等分分裝為一次實驗使用量，分裝后，-80℃凍存