HITRAP CAPTO Q-色譜柱 -耗材-生物在線
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HITRAP CAPTO Q

HITRAP CAPTO Q

商家詢價

產品名稱： HITRAP CAPTO Q

英文名稱： HITRAP CAPTO Q

產品編號： 11-0013-02

產品價格： 0

產品產地： USA

品牌商標： 美國GE

更新時間： null

使用范圍： null

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11-0013-02 HITRAP CAPTO Q, 5X1ML
11-0013-03 HITRAP CAPTO Q, 5X5ML
11-0032-73 HITRAP CAPTO MMC, 5X1ML
11-0032-75 HITRAP CAPTO MMC, 5X5ML
11-0034-93 HITRAP MABSELECT SURE, 5X1ML
11-0034-94 HITRAP MABSELECT SURE, 1X5ML
11-0034-95 HITRAP MABSELECT SURE, 5X5ML
17-0402-01 HITRAP PROTEIN A HP, 5 X 1 ML
17-0402-03 HITRAP PROTEIN A HP,2 X 1 ML
17-0403-01 HITRAP PROTEIN A HP,1 X 5 ML
17-0403-03 HITRAP PROTEIN A HP, 5 X 5 ML
17-0404-01 HITRAP PROTEIN G HP, 5 X 1 ML
17-0404-03 HITRAP PROTEIN G HP, 2 X 1 ML
17-0405-01 HITRAP PROTEIN G HP,1 X 5 ML
17-0405-03 HITRAP PROTEIN G HP, 5 X 5 ML
17-0406-01 HITRAP HEPARIN HP, 5 X 1 ML
17-0407-01 HITRAP HEPARIN HP,1 X 5 ML
17-0407-03 HiTrap Heparin HP 5x5ml

Capto IEX Media/HiTrap Capto IEX Columns

  • High-throughput medium for capture and intermediate purification at process-scale.
  • Combined high binding capacity, high flow rate, and low backpressure properties reduce process cycle times and improve productivity.
  • Rigid agarose matrix enables high bed heights and purification of viscous samples at high flow rates.
  • Flow rates of up to 700 cm/h can be employed at process-scale with a backpressure below 3 bar.
  • HiTrap? prepacked column format for easy screening and convenient process development.

Capto IEX Media/HiTrap Capto IEX Columns

Technical Information


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Capto? Q has a strong quaternary ammonium (Q) anion exchanger, Capto DEAE has a weak diethylaminoethyl anion exchanger, and Capto S has a strong sulfoethyl cation exchanger coupled to a chemically modified, high-flow agarose matrix. The modified agarose matrix provides greater particle rigidity without compromising pore size, which allows more flexibility in process-scale applications. High dynamic binding capacity is achieved with a dextran surface extender that coats the agarose matrix.

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Capto? ViralQ* is in all respects identical to Capto Q, except that a license for use of the product for virus purification is included.

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Prepacked HiTrap? Capto? Q, HiTrap Capto DEAE (strong and weak anion exchange, respectively), and HiTrap? Capto? S (strong cation exchange) columns are ideal for small scale purifications. HiScreen columns prepacked with the same media are excellent tools for method optimization in process development, such as screening of selectivity, binding and elution conditions.

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* See licensing information at back of catalog.

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Capto ion exchangers increase productivity and shorten overall process time
Stationary phase Process cycle time (min) Productivity (kg/m3/h)
Capto? DEAE? 197 37
DEAE Sepharose Fast Flow? 306 12
Capto? Q* 91 11
Q Sepharose? Fast Flow* 190 3
Capto S? 131 53
SP Sepharose? Fast Flow? 229 17
* Productivity calculations are based on purification of GFP in E. coli homogenate.
? Productivity calculations are based on purification of a-chymotrypsin in E. coli homogenate.
? Productivity calculations are based on pure amyloglucosidase.

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TECHNICAL SPECIFICATIONS
Ion exchanger type? ?
Capto Q? Quaternary ammonium, strong anion?
Capto DEAE? Diethylaminoethyl, weak anion?
Ionic capacity? ?
Capto Q? 0.16-0.22 mmol Cl-/ml?
Capto S? 0.11-0.14 mmol Na+/ml?
Capto DEAE? 0.29?0.35 mmol Cl-/ml?
Average particle size? 90 μm?
Matrix? Highly cross-linked agarose with dextran surface extender?
Dynamic binding capacity?
Capto Q? Min. 100 mg BSA/ml medium capacity at ?10% breakthrough, 1-min residence time?
Capto S? Min. 120 mg lysozyme/ml medium capacity? at 10% breakthrough, 1-min residence time?
Capto DEAE? Min. 90 mg ovalbumin/ml medium at 10% breakthrough, 1-min residence time?
Pressure/flow spec.? 3 bar at 700 cm/h, 1-m diameter column, ?20-cm bed height in water?
pH stability? ?
Capto Q? 2-14 (short term), 2-12 (long term)?
Capto S? 3-14 (short term), 4-12 (long term)?
Capto DEAE? 2-14 (short term), 2-12 (long term)?
Chemical stability ? Stable in all commonly used aqueous buffers: 8 M urea, 6 M Gua-HCl, 70% ethanol, 1 M NaOH and 1 M acetic acid.?
Storage? 20% ethanol (Q, DEAE), 0.2 M sodium acetate in 20% ethanol (S)?
Storage temperature? 4°C to 30°C?
HiTrap prepacked columns
Column volume? 1 ml and 5 ml?
Column dimensions*? 0.7 × 2.5 cm (1 ml); 1.6 × 2.5 cm (5 ml)?
Max. pressure?? 3 bar (0.3 MPa)?
* Given the short bed height of the HiTrap? column, the flow rate should not exceed 150?cm/h to achieve 1-min residence time for high dynamic binding capacity.
? H2O at 25°C