Product: Anti-Caspase-3, Clone AM1-4
Cat. No: MC-460 (100 μg)
Background:
Caspase-3 along with caspase 7 and 6 form a
group of effector caspases that are responsible
for the cleavage of multiple substrates including
the cytyokeratins, PARP, alpha fodrin, NuMA
and others. Caspase-7 occurs in three variant
forms.
Caspase-3-like activities are required for Fasmediated
apoptosis. However, the role of
caspase-1 and caspase-3 in mediating Fasinduced
cell death is not clear. Although wildtype,
caspase-1(-/-), and caspase-3(-/-)
hepatocytes were killed at a similar rate when
co-cultured with FasL expressing NIH 3T3 cells,
caspase-3(-/-) hepatocytes displayed drastically
different morphological changes as well as
significantly delayed DNA fragmentation
observed in hepatocytes. Cleavage of various
caspase substrates implicates apoptotic events,
including gesolin, fodin, lamin B, and
DFF45/ICAD are delayed or absent. The altered
cleavage of these key substrates is likely
responsible for the aberrant apoptosis observed
in both hepatocytes and thymocytes deficient in
caspase-3.
Species Reactivity:
Human, others not tested.
Ig Isotype: mouse IgG1
Immunogen:
Hybridoma produced by the fusion of
splenocytes from mice immunized with
recombinant human Caspase-3 protein and
mouse myeloma cells.
Format:
Mouse monoclonal antibody against human
Caspase-3 (cysteine-requiring aspartate
protease-3). Available in 100 ul vials at a
concentration of 1 mg/ml (100ug) in PBS with
0.08% sodium azide. Purified and 0.2 μm sterile
filtered.
Storage and Stability:
Stable for 1 week at 2-8°C. Stable for 24 months
when stored at -20°C. Aliquot to avoid
freeze/thaw cycles.
Applications and Suggested Dilutions:

Western Blot
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product