Size??100ug
Cat code??200054
Species Cross-reactivity??H
Key Application??WB,ICC
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG2a
Molecular Weight??220kDa
Sensitivity??This antibody detects endogenous levels of KDM5C and does not corss-react with related proteins
Immunogen??Purified recombinant human KDM5C (C-terminus) protein fragments expressed in E.coli
UniProt Number??P41229
GeneBank ID??NM_004187.3
Subcellular location??Nucleus
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername??MRXJ; SMCX; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E
Gene_symbol??MRXJ; SMCX; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E
Summary??This gene is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. The DNA-binding motifs suggest this protein is involved in the regulation of transcription and chromatin remodeling. Mutations in this gene have been associated with X-linked mental retardation. Alternative splicing results in multiple transcript variants.
UniPort summary  Function??Histone demethylase that specifically demethylates ′Lys-4′ of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 ′Lys-9′, H3 ′Lys-27′, H3 ′Lys-36′, H3 ′Lys-79′ or H4 ′Lys-20′. Demethylates trimethylated and dimethylated but not monomethylated H3 ′Lys-4′. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements.
Application Images :??
Western blot detection of KDM5C(C-terminus) antibody in Hela&293 cell lysates using KDM5C(C-terminus) antibody (1:1000 diluted).
Predicted band size: 176KDa
Observed band size: 220KDa
Immunocytochemistry of HeLa cells using anti- KDM5C (C-terminus) antibody diluted 1:150.