Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

Using Medium 199:
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 510% CO2 environment to maintain physiological pH. Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Dual-Site cGMP Manufacturing:
Gibco Medium 199 is manufactured in a cGMP New York facility that is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 . For supply chain continuity, we offer an identical Gibco Medium 199 product made in the Scotland facility (31100-019). This second cGMP manufacturing location is also registered with the FDA as a medical device manufacturer and is certified to ISO 13485.