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細胞名稱：人原髓細胞白血病細胞（STR鑒定正確）

細胞別稱：HL 60; HL.60; HL60

該細胞由CollinsSJ從一位患有急性早幼粒細胞性白血病的36歲白人女性的外周血中分離建立；可自發分化，或在丁酸鹽、次黃嘌呤、佛波醇肉豆蔻酸(PMA,TPA)、DMSO(1%to1.5%)、放線菌素D和視黃酸的刺激下發生分化；PMA刺激后可分泌TNF-α。該細胞具有吞噬活性和趨化反應，癌基因myc陽性，表達補體受體和FcR。

細胞名稱：人原髓細胞白血病細胞

細胞簡稱：HL-60

產品貨號：TCH-C204

種屬來源：人

組織來源：外周血

疾病特征：急性粒-單核細胞白血病

細胞形態：髓母細胞樣

生長特性：懸浮生長

培養體系：RPMI-1640+20%FBS+1%P/S

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎培養基+30%FBS+10%DMSO，液氮儲存

質量檢測：細菌、真菌、支原體檢測均為陰性

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