重組小鼠BMP-4蛋白（Recombinant Murine BMP-4 Protein）

貨 號：R-TL680

產品描述（Description）

BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair. Mature mouse and human BMP-4 share 98% aa sequence identity. Mouse BMP-4 shares 85% aa sequence identity with mouse BMP-2 and 35%-54% with other mouse BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation. In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans.

同義詞（Alternative Names）

Bmp-4 Protein,Mouse、Bmp2b Protein,Mouse、Bmp2b-1 Protein,Mouse、Bmp2b1 Protein

蛋白序列（Protein Sequence）

DNA 序列編碼小鼠 BMP-4（ NCBI Reference Sequence: NP_031580.2）表達帶有 Fc 標簽在 C 末端

分子量（Molecular Weight）

重組小鼠 BMP-4蛋白 包含 339 個氨基酸，預測理論分子量為 38.9 kD

純度（Purity）

>90%，采用 SDS-PAGE 凝膠分析

內毒素（Endotoxin）

<1EU/μg

表達宿主（Expression host）

HEK293 細胞

生物活性（Biological Activity）

通過 ATDC-5 細胞誘導堿性磷酸酶的產生來確定其活性。預期的 ED??為 10-30ng/mL

純化方式（ Purification Method ）

層析純化

性狀（Character）

白色疏松體

使用說明（Instructions）

凍干制劑可在 2~8℃保存 24 個月。 

如需分裝，可用注射用水、生理鹽水、培養基或 PBS 溶解，溶解后分裝成小份，置于-20℃保存 期 6 個月，-80℃保存期 12 個月，稀釋后濃度不低于 100μg/mL。避免反復凍融。

  注意：For research and manufacturing use.