Anti-AKT1 (E49K) Mouse Monoclonal Antibody-抗體-抗體-生物在線
武漢紐斯特生物技術有限公司
Anti-AKT1 (E49K) Mouse Monoclonal Antibody

Anti-AKT1 (E49K) Mouse Monoclonal Antibody

商家詢價

產品名稱： Anti-AKT1 (E49K) Mouse Monoclonal Antibody

英文名稱： Anti-AKT1 (E49K) Mouse Monoclonal Antibody

產品編號： 26137

產品價格： null

產品產地： 美國

品牌商標： NewEastBio

更新時間： null

使用范圍： ELISA、WB、 IHC

武漢紐斯特生物技術有限公司
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AKT1 (E49K) [26137]
[100ul]		 ?

Catalog Number:??26137
Gene Symbol:??AKT1; AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Description: ??Anti-AKT1 (E49K) Mouse Monoclonal Antibody
Background: ??AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinases, which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Its function is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. There are evidences that mutation or abnormal expression in AKT1 is associated with cancers.
Immunogen: ??A synthetic peptide from the internal region of AKT1 which includes the mutation of E49K, human origin.
Applications: ??ELISA, WB, IHC
Recommended Dilutions: ??
ELISA: 1:1000-1:5000?
WB: 1:500-1:2000?
IHC: 1:50-1:100
Concentration: ??1 mg/ml
Host: ??Mouse
Format: ??Liquid
Clonality: ??Monoclonal
Isotype: ??
Purity:???Purified from ascites
Storage buffer:
Preservative:??no
Constituents:??PBS (without Mg2+?and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:??Recognizes E49K mutant, but not wild-type AKT1 of vertebrates.
Storage Conditions:???Store at -20°C. Avoid freeze / thaw cycles
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Western blot:??
Western blot analysis of recombinant AKT1 (E49K) and wildtype proteins. Purified His-tagged AKT1 (E49K) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with anti-AKT1 (E49K) monoclonal antibody (Cat. #26137).
Immunofluorescence: ??
Immunofluorescence of cells expressing AKT1 proteins with anti-AKT1 (E49K) antibody. HEK293T cells were transfected with pCDNA3-GFP-AKT1 (WT) plasmid (left column) or pCDNA3-GFP-AKT1 (E49K) plasmid (right column), then fixed and stained with anti-AKT1 (E49K) monoclonal antibody (Cat. #26137).