GST monoclonal antibody, clone 1E5
產品名稱： GST monoclonal antibody, clone 1E5
英文名稱： GST monoclonal antibody, clone 1E5
產品編號： MAB1132
產品價格： null
產品產地： 臺灣
品牌商標： Abnova
更新時間： null
使用范圍：
亞諾法生技股份有限公司（Abnova)
- 聯系人 :
- 地址 : 臺灣臺北市內湖區洲子街 108 號 9 樓
- 郵編 : 11493
- 所在區域 : 臺灣
- 電話 : +886-920**1152 點擊查看
- 傳真 : 點擊查看
- 郵箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against full length recombinant GST.
- Immunogen:
- Recombinant protein corresponding to full length GST .
- Host:
- Mouse
- Reactivity:
- Human
- Form:
- Liquid
- Isotype:
- IgG2b, kappa
- Storage Buffer:
- In PBS, pH 7.4 (0.1% sodium azide)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Conventional immunoassays underestimate anti-GST antibody titre.
Cartwright GA, Rothel JS, Lightowlers MW.J Immunol Methods. 1995 Feb 13;179(1):31-5.
- 2.
- A non-radioactive assay for the detection and quantitation of a DNA binding protein.
Hibma MH, Ely SJ, Crawford L.Nucleic Acids Res. 1994 Sep 11;22(18):3806-7.
- 3.
- Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase.
Smith DB, Davern KM, Board PG, Tiu WU, Garcia EG, Mitchell GF.Proc Natl Acad Sci U S A. 1986 Nov;83(22):8703-7.
- Applications
- Western Blot (Recombinant protein)
- Western blot analysis of recombinant GST (28 kDa) and GST - fusion protein (61 kDa) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with GST monoclonal antibody, clone 1E5 (1 : 1000) (Cat # MAB1132) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system. Arrows indicate GST and GST - fusion proteins (20 ng) .