Size??100ul
Cat code??220011
Species Cross-reactivity??H,R,M,Mk
Key Application??WB,ICC
Host??Mouse
Clone Number??7G6-C5-D8
Antibody type??Monoclonal antibody
Purified method??
Isotype??IgG2a
Molecular Weight??48.8kDa
Sensitivity??The antibody detects endogenous level of total HDAC3 protein. The antibody detects endogenous level of total HDAC3 protein.
Immunogen??Purified recombinant fragment of human HDAC3 (aa224-428) expressed in E. Coli.
UniProt Number??O15379
GeneBank ID??NM_003883
Subcellular location??Nucleus.
Formulation??Ascitic fluid containing 0.03% sodium azide.
Altername??HD 3 ; HD3 ; HDAC 3 ; HDAC3 ; HDAC3_HUMAN ; Histone deacetylase 3 (HD3) (RPD3-2) ; histone deacetylase 3 ; RPD 3 ; RPD3 2 ; RPD3 ; RPD3-2 ; SMAP 45 ; SMAP45 ;
Gene_symbol??HD3; RPD3; RPD3-2
Summary??Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]
UniPort summary  Function??Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation
Application Images :??
Western blot detection of HDAC3 antibody in COS7, C6, Hela and 3T3 cell lysates using HDAC3 antibody(dilution 1:500).Predicted band size:48.8KDa.Observed band size:48.8KDa.
Immunocytochemistry of HeLa cells using anti-HDAC3 antibody diluted 1:100.