LightCycler?EvoScript?RNA SYBR?Green I Master

The LightCycler? EvoScript RNA SYBR? Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.

LightCycler ? ?EvoScript RNA SYBR ? ?Green I Master專為敏感、高特異性、高精度一步RT-qPCR而設計，特別適用于基因表達分析。?

For general laboratory use.供一般實驗室使用。

Product No：07800134001???產品編號：07800134001 ?

Pack Size：200 x 20 μL?reactions ?包裝尺寸：200 x 20 μL?反應

Product No：07800177001 ?產品編號：07800177001 ??

Pack Size：1000 x 20 μL?reactions ??包裝尺寸：1000 x 20 μL 反應

Keywords: SYBR Green??關鍵詞：SYBR 綠色

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概述：

The LightCycler? EvoScript RNA Probes Master (1-vial composition) is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.

LightCycler?EvoScript RNA Probes Master（1 瓶組合物）專為靈敏、高特異性、高精度的一步法 RT-qPCR 而設計，尤其適用于基因表達分析。

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Ease of use, convenience, speed, reduced error

Result consistency, stability and tolerance of targets of varying secondary structure

Ability to detect low copy or rare transcripts for gene expression applications

Superior quantification capability for gene expression applications

Easier assay optimization

Contamination control capability

Generate consistent and robust results, no matter what sample type you are starting from or what target you are detecting

Rely on a higher sensitivity when detecting low copy numbers, with the input of larger sample volumes into the reaction

Obtain fast and specific results

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§?適用簡單、方便快捷、減少錯誤

§?結果表明，不同二級結構的目標具有一致性、穩定性和耐受性

§?能夠檢測低拷貝或罕見的轉錄本的基因表達應用

§?卓越的基因表達應用定量能力

§?更容易分析優化

§?污染控制能力

§?生成一致且可靠的結果，無論您從什么樣本類型開始或檢測什么指標

§?在檢測低拷貝數時依靠更高的靈敏度，將更大的樣品體積輸入到反應中

§?獲得快速和具體的結果

Contents

Master Mix, 5× conc. Contains enzymes for reverse transcription and PCR, RT-qPCR Reaction Buffer, dATP, dCTP, dGTP, and dUTP, Mg(OAc)2, SYBR? Green I dye and proprietary additives

07 800 134 001 ??2 vials, 440 μL each

07 800 177 001 ?10 vials, 440 μL each

Water, PCR grade To adjust the final reaction volume.

07 800 134 001 ??3 vials, 1 mL each

07 800 177 001 ??2 vials, 7.25 mL each

內容

§?包含用于逆轉錄和 PCR 的酶、RT-qPCR 反應緩沖液、dATP、dCTP、dGTP 和 dUTP、Mg(OAc) 2、SYBR ? ?Green I 染料和專有添加劑

§?07 800 134 001 ???2瓶，每瓶 440 μL
07 800 177 001 ???10瓶，每瓶440μL

§?水，PCR 級 調整最終反應體積。
07 800 134 001 ???3瓶，每瓶 1 mL
07 800 177 001???2瓶，每瓶 7.25 mL

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應用：

The LightCycler? EvoScript RNA SYBR? Green I Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.

The 1-vial composition is ideally suited for easy reaction assembly, requiring only the addition of primers and target RNA. The proprietary reaction buffer allows a convenient hot start RT-qPCR.

LightCycler ? ?EvoScript RNA SYBR ? ?Green I Master 專為靈敏、高特異性、高精度的一步法 RT-qPCR 而設計，尤其適用于基因表達分析。1瓶組合物非常適合簡單的反應組裝，只需要添加引物和目標 RNA。專有的反應緩沖液允許方便的熱啟動 RT-qPCR。

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In addition, the enzymes for reverse transcription and for PCR feature hot start for premium specificity1).

The master mix is optimized for SYBR? Green I applications and does not require optimization with Mg(OAc)2.

1)The reverse transcription activity will be suppressed at temperatures significantly below 60°C.

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此外，用于逆轉錄和 PCR 的酶具有熱啟動特性，具有卓越的特異性1)。

預混液針對 SYBR ? ?Green I 應用進行了優化，不需要使用 Mg(OAc) 2進行優化。

1)在顯著低于 60°C 的溫度下，逆轉錄活性將被抑制。