Size??100ul
Cat code??220010
Species Cross-reactivity??H
Key Application??WB
Host??Mouse
Clone Number??1B2-E5-G3
Antibody type??Monoclonal antibody
Purified method??
Isotype??IgG1
Molecular Weight??93kDa
Sensitivity??The antibody detects endogenous level of total KDM1 protein.
Immunogen??Purified recombinant fragment of human LSD1 expressed in E. Coli.
UniProt Number??O60341
GeneBank ID??
Subcellular location??Nucleus.
Formulation??Ascitic fluid containing 0.03% sodium azide.
Altername??Amine oxidase (flavin containing) domain 2 ; Amine oxidase flavin containing domain 2 ; Amine oxidase flavin containing domain protein 2 ; AOF 2 ; AOF2 ; BHC110 ; BRAF35 HDAC complex protein BHC110 ; BRAF35-HDAC complex protein BHC110 ; FAD binding protein BRAF35 HDAC complex, 110 kDa subunit ; Flavin containing amine oxidase domain containing protein 2 ; Flavin-containing amine oxidase domain-containing protein 2 ; KDM 1 ; KDM1 ; Kdm1a ; KDM1A_HUMAN ; KIAA0601 ; LSD 1 ; LSD1 ; Lysine (K) specific demethylase 1 ; Lysine (K) specific demethylase 1A ; Lysine specific histone demethylase 1 ; Lysine specific histone demethylase 1A ; Lysine-specific histone demethylase 1 ; Lysine-specific histone demethylase 1A ;
Gene_symbol??AOF2; KDM1; LSD1; BHC110
Summary??This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
UniPort summary  Function??Histone demethylase that demethylates both ′Lys-4′ (H3K4me) and ′Lys-9′ (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of ′Thr-6′ of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated ′Lys-370′ of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7.
Application Images :??
Western blot detection of KDM1 antibody in A549 and Jurkat cell lysates using KDM1 antibody(dilution 1:500).Predicted band size:93 KDa.Observed band size:93KDa.