Anti-Rat SCF
Catalog Number:
500-P202
Preparation:
Produced from sera of rabbits immunized with highly pure recombinant Rat SCF. Anti-Rat SCF specific antibody was purified by affinity chromatography employing an immobilized Rat SCF matrix.
Immunogen:
E.coli derived Recombinant Rat SCF (PeproTech catalog #400-22)
Application:
ELISA
Sandwich
To detect rat SCF by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat SCF (500-P202Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant rat SCF. 

ELISA: Sandwich
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Western Blot
To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. 

Western Transfer (Western Blot)
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Neutralization
To yield one-half maximal inhibition [ND50] of the biological activity of rat SCF (50.0 ng/ml), a concentration of 1.2-1.8 μg/ml of this antibody is required. 
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Research Interests:
Cell Culture
Proliferation
Stem Cells & Differentiation
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Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.