Anti-53BP2/ASPP2抗體[EPR13837]-抗體-抗體-生物在線
深圳市宇德立生物科技有限公司
Anti-53BP2/ASPP2抗體[EPR13837]

Anti-53BP2/ASPP2抗體[EPR13837]

商家詢價

產品名稱： Anti-53BP2/ASPP2抗體[EPR13837]

英文名稱： 53BP2 antibody Apoptosis stimulating of p53 protein 2 antibody Apoptosis stimulating protein of p53 2 antibody Apoptosis-stimulating of p53 protein 2

產品編號： ab181377

產品價格： null

產品產地： 英國

品牌商標： abcam

更新時間： null

使用范圍：

深圳市宇德立生物科技有限公司
  • 聯系人 :
  • 地址 : 深圳市寶安區西鄉寶民二路賢基大廈4E
  • 郵編 :
  • 所在區域 : 廣東
  • 電話 : 133****4454 點擊查看
  • 傳真 : 點擊查看
  • 郵箱 : 1484332550@qq.com

?

  • 經測試應用WB,?IHC-P,?ICC/IFmore details
  • 種屬反應性
    與反應:?Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human 53BP2/ASPP2 aa 50-150. The exact sequence is proprietary.
    Database link:?Q13625

  • 陽性對照
    • Jurkat, Saos-2, MCF7 cell lysate; Human kidney tissue; Human testis tissue
  • 常規說明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb??technology. RabMAb??technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

  • 形式Liquid
  • 存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存儲溶液pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 0.05% BSA, 50% Glycerol
  • 純度Tissue culture supernatant
  • 克隆單克隆
  • 克隆編號EPR13837
  • 同種型IgG
  • 研究領域

應用

Our?Abpromise guarantee?covers the use of?ab181377?in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

應用 Abreviews 說明
WB ? 1/10000 - 1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).
IHC-P ? 1/100 - 1/250.
ICC/IF ? 1/50 - 1/100.
  • 應用說明Is unsuitable for Flow Cyt or IP.

靶標

  • 功能Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
  • 組織特異性Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
  • 序列相似性Belongs to the ASPP family.
    Contains 2 ANK repeats.
    Contains 1 SH3 domain.
  • 結構域The ankyrin repeats and the SH3 domain are required for a specific interactions with TP53.
  • 細胞定位Cytoplasm > perinuclear region. Nucleus. Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.

  • Anti-53BP2/ASPP2 antibody [EPR13837] 圖像

    • All lanes :?Anti-53BP2/ASPP2 antibody [EPR13837] (ab181377) at 1/10000 dilution

      Lane 1 :?Jurkat cell lysate
      Lane 2 :?Saos-2 cell lysate
      Lane 3 :?MCF7 cell lysate

      Lysates/proteins at 10 μg per lane.


      Predicted band size :?126 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling 53BP2/ASPP2 with ab181377 at a 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling 53BP2/ASPP2 with ab181377 at a 1/100 dilution.

    • Immunofluorescent analysis of?Jurkat cells?labeling 53BP2/ASPP2 with ab181377 at a 1/50 dilution.