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Description:
The expression of foreign proteins at high levels in E. coli often results in the formation of inclusion bodies composed of insoluble aggregates of the expressed protein. The inclusion bodies are recovered from bacterial lysates by centrifugation and are washed with Triton X-100 and EDTA to remove as much bacterial protein as possible from the aggregated foreign protein. To obtain soluble protein, the washed inclusion bodies are dissolved in denaturing agents and the released protein is then refolded by gradual removal of the denaturing reagents by dilution or dialysis. The kit has been used in extraction of many proteins. However, each protein may require a slightly different procedure, which must be determined empirically.
Applications: This Kit provides reagents to extract protein aggregated in inclusion body.
Features:Simple & Fast.;High yield of the inclusion body protein.
Storage Condition:Store at 4℃;? DNase I, Lysozyme and protease inhibitor should be stored at -20℃.