包涵體蛋白抽提試劑盒
產品名稱： 包涵體蛋白抽提試劑盒
英文名稱： Inclusion Body Protein Extaction Kit
產品編號： BS687-for 30g bacteria
產品價格： 0
產品產地： Canada
品牌商標： BBI
更新時間： null
使用范圍： null
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Description:
The expression of foreign proteins at high levels in E. coli often results in the formation of inclusion bodies composed of insoluble aggregates of the expressed protein. The inclusion bodies are recovered from bacterial lysates by centrifugation and are washed with Triton X-100 and EDTA to remove as much bacterial protein as possible from the aggregated foreign protein. To obtain soluble protein, the washed inclusion bodies are dissolved in denaturing agents and the released protein is then refolded by gradual removal of the denaturing reagents by dilution or dialysis. The kit has been used in extraction of many proteins. However, each protein may require a slightly different procedure, which must be determined empirically.
Applications: This Kit provides reagents to extract protein aggregated in inclusion body.
Features:Simple & Fast.;High yield of the inclusion body protein.
Storage Condition:Store at 4℃;? DNase I, Lysozyme and protease inhibitor should be stored at -20℃.