基本信息

產品名稱Recombinant human FGF1 protein英文名稱Recombinant human FGF1 protein規格或純度Animal Free, >95% SDS-PAGE, Active, E.coli, No tag, 16-155 aa生物活性Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line in the presence of heparin. The ED?? for this effect is typically <0.5ng/mL.內毒素水平< 0.1 EU/μg種屬Human氨基酸Phe16-Asp155序列FNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAES VGEVYIKSTE TGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVG LKKNGSCKRG PRTHYGQKAILFLPLPVSSD蛋白標簽No tag蛋白長度Full length proteinCarrier FreeYesAnimalFreeYesAccession#P05230來源Recombinant預測分子量15.9 kDaSDS-PAGE13.3 kDa, under reducing conditions; 13.3 kDa, under non-reducing conditions.

相關屬性

特點與好處·Application:FuncS,SDS-PAGE,WB物理外觀Lyophilized儲存緩沖液Lyophilized from a sterile solution containing 50 mM Tris.Cl, pH7.5, 150mM NaCl.復溶After receiving lyophilized powder protein, centrifuge first, and then r econstitute at 100μg/mL in sterile PBS.儲存溫度-20°C儲存運輸條件超低溫冰袋運輸穩定性與儲存Stable for 12 months from the date of receipt of the product under proper storage andhandling conditions. Avoid repeated freeze-thaw cycles.

常規信息

基因名FGF1基因 ID2246細胞定位Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SY序列相似性Belongs to the heparin-binding growth factors family.通路MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton基因別名AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-1, FGF-alpha, FGFA, GLIO703, HBGF-1, HBGF1英文別名Acidic fibroblast growth factor aFGF Beta; endothelial cell growth factor Beta; endothelial cell growth factor; ECGF; ECGF beta; ECGF-beta; ECGFA; ECGFB; Endothelial Cell Growth Factor alpha; Endothelial Cell Growth Factor beta; FGF 1; FGF alpha; Fgf1; FG別名Acidic fibroblast growth factor aFGF Beta; endothelial cell growth factor Beta; endothelial cell growth factor; ECGF; ECGF beta; ECGF-beta; ECGFA; ECGFB; Endothelial Cell Growth Factor alpha; Endothelial Cell Growth Factor beta; FGF 1; FGF alpha; Fgf1; FG

一般描述

Purity

>95% as determined by SDS-PAGE and Coomassie blue staining

Function

The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.

Sequence similarities

Belongs to the heparin-binding growth factors family.

Cellular localization

Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.

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Protein Bioactivity: Recombinant Human FGF1 Protein (rp145907)

Recombinant Human FGF1 Protein (rp145907)-Protein Bioactivity

Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line in the presence of heparin. The ED50 for this effect is typically <0.5ng/mL.

SDS-PAGE: Recombinant Human FGF1 Protein (rp145907)

Recombinant Human FGF1 Protein (rp145907)-SDS-PAGE

3μg/lane of Recombinant Human FGF1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie? Blue staining, showing a single band at 13.3 kDa.