Size??100ug
Cat code??200532
Species Cross-reactivity??H,M
Key Application??WB
Host??Mouse
Antibody type??Monoclonal antibody
Purified method??Affinity purified
Isotype??IgG2b
Molecular Weight??95kDa
Sensitivity??This antibody detects endogenous levels of BRAF  and does not cross-react with related proteins.
Immunogen??Purified recombinant human BRAF  protein fragments expressed in E.coli.
UniProt Number??P15056
GeneBank ID??NM_004333
Subcellular location??Nucleus.Cytoplasm.Cell membrane.
Formulation??Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.2% sodium azide,0.1mg/mlBSA and 50% glycerol.
Altername??FLJ95109;94 kDa B raf protein;B raf 1;B Raf proto oncogene serine threonine protein kinase;BRAF 1;BRAF;BRAF_HUMAN;BRAF1;cRmil;FLJ95109;MGC126806;MGC138284;Murine sarcoma viral (v-raf) oncogene homolog B1;Murine sarcoma viral v raf oncogene homolog B1;NS7;p94;Proto-oncogene B-Raf;Proto-oncogene c-Rmil;RAFB 1;RAFB1;Serine/threonine-protein kinase B-raf;v raf murine sarcoma viral oncogene homolog B1;v-Raf murine sarcoma viral oncogene homolog B1.
Gene_symbol??NS7; BRAF1; RAFB1; B-RAF1
Summary??This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
UniPort summary  Function??Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.
Application Images :??
Western blot detection of BRAF in Hela,Ramos,K562,C2C12 & Jurkat cell lysates using BRAF antibody (1:300 diluted).Predicted band size: 86KDa.Observed band size: 95KDa.