重組人ICOS蛋白（Recombinant Human ICOS Protein），GMP級

產品貨號：GMP-TL723

產品描述（Description）

Inducible T-cell costimulator (ICOS) is also known as Activation-inducible lymphocyte immunomediatory molecule (AILIM), CD278, which belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. ICOS enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. CD278 / ICOS prevents the apoptosis of pre-activated T-cells and also plays a critical role in CD40-mediated class switching of immunoglobin isotypes.

分子別名（Synonym）

CD278、AILIM、Inducible T-cell costimulator

表達宿主（Expression host）

HEK293細胞

來源（Source）

Human

蛋白序列（Protein Sequence）

DNA序列編碼人ICOS（NCBI Reference Sequence: NP_036224.1）表達帶有Fc標簽在C末端

分子量（Molecular Weight）

重組人ICOS蛋白 包含354個氨基酸，預測理論分子量為40.7kD

純度（Purity）

≥95%采用SDS-PAGE凝膠和高效液相色譜分析

內毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定人 B7-H2(His 標簽)2ug/mL (100uL/孔) 能夠結合 ICOS(Fc 標簽)的線性范圍是 0.2-10 ng/mL

穩定性（Stability）

凍干的樣本的可以在4℃保存24個月，溶解后的液體可以于-20℃保存6-12個月，并且避免反復凍融

組成（Component）

含有6%甘露醇的無菌PBS（PH 7.4）的凍干粉

參考文獻

1、Differentiation of ICOS+ and ICOS? recent thymic emigrant regulatory T cells (RTE Tregs) during normal pregnancy, pre‐eclampsia and HELLP syndrome M. I. Wagner, M. J?st, J. Spratte, M. Schaier, K. Mahnke, S. Meuer, M. Zeier, A. Steinborn Clin Exp Immunol. 2016 Jan; 183(1): 129–142. Published online 2015 Oct 12. doi: 10.1111/cei.12693 

2.、ICOS Co-Stimulation: Friend or Foe? Daniel J. Wikenheiser, Jason S. Stumhofer Front Immunol. 2016; 7: 304. Published online 2016 Aug 10. doi: 10.3389/fimmu.2016.00304 

3、Loss of Immune Tolerance Is Controlled by ICOS in Sle1 MiceNanette Mittereder, Ellen Kuta, Geetha Bhat, Karma Dacosta, Lily I. Cheng, Ronald Herbst, Gianluca Carlesso J Immunol. 2016 Jul 15; 197(2): 491–503. Published online 2016 Jun 13. doi: 10.4049/jimmunol.1502241 

4、Development of interleukin-17-producing Vγ2+ γδ T cells is reduced by ICOS signaling in the thymus Terkild Brink Buus, Jonas Damg?rd Schmidt, Charlotte Menné Bonefeld, Carsten Geisler, Jens Peter Holst Lauritsen Oncotarget. 2016 Apr 12; 7(15): 19341–19354. Published online 2016 Mar 29. doi: 10.18632/oncotarget.8464

  注意：For research and manufacturing use.