HITRAP HEPARIN HP
產品名稱： HITRAP HEPARIN HP
英文名稱： HITRAP HEPARIN HP
產品編號： 17-0406-01
產品價格： 0
產品產地： USA
品牌商標： 美國GE
更新時間： null
使用范圍： null
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| 17-0406-01 | HITRAP HEPARIN HP, 5 X 1 ML |
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| 17-0716-01 | HITRAP NHS-ACTIV HP, 5 X 1ML |
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HiTrap? Heparin HP Columns
- Prepacked with Heparin Sepharose? High Performance for purification of proteins such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.
- Stable over pH 5-10 and tolerates all commonly used aqueous buffers.
HiTrap? Heparin HP Columns
Technical Information
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Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand; e.g. in its interaction with coagulation factors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases. Heparin is coupled to the Sepharose? High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap? Heparin is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap? Heparin columns equilibrated with 25 ml 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily done with a syringe and the supplied luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4 to 30°C.
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| TECHNICAL SPECIFICATIONS | |
| Ligand? | Porcine heparin? |
| Ligand coupling method? | Reductive amination? |
| Ligand density? | ? 10 mg heparin/ml medium? |
| Matrix? | Highly cross-linked agarose, 6%? |
| Average particle size? | 34 μm? |
| pH stability? | pH 5-10? |
| Storage? | 20% ethanol? |
| Storage temperature? | 4°C to 30°C? |
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Purification of Antithrombin III from bovine plasma.
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SDS -PAGE electrophoresis with PhastSystem? and PhasGel Gradient 8-25, silver stained