英文名稱
AIF1 (9A3)
中文名稱
離子鈣接頭蛋白單克隆抗體
別????名
AIF 1; AIF1; AIF1 protein; IBa1; iba-1; IBa 1; allograft inflammatory factor 1; Allograft inflammatory factor 1 splice variant G; balloon angioplasty responsive transcription; BART 1; G1; G1 putative splice variant of allograft inflamatory factor 1; IBA 1; IBA1; interferon gamma responsive transcript; Ionized calcium binding adapter molecule 1; ionized calcium-binding adapter molecule; IRT 1; IRT1; Microglia response factor; MRF1; Protein G1.??
研究領域
細胞生物??免疫學??神經生物學??
抗體來源
Rabbit
克隆類型
Monoclonal
克?隆?號
9A3
交叉反應
Human,?Mouse,?Rat,?
產品應用
WB=1:500?IP=1:10-50?IHC-P=1:100-500?ICC=1:50-100?IF=1:50-100?（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分?子?量
17kDa
細胞定位
細胞漿?細胞膜?
性????狀
Liquid
濃????度
1mg/ml
免?疫?原
KLH conjugated synthetic peptide derived from human AIF1:100-147/147?
亞????型
IgG
純化方法
affinity purified by Protein A
儲?存?液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed
PubMed
產品介紹
Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.

Function:
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Subunit:
Homodimer (Potential). Monomer. Interacts with LCP1.

Subcellular Location:
Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.

Tissue Specificity:
Detected in T-lymphocytes and peripheral blood mononuclear cells.

Similarity:
Contains 2 EF-hand domains.

SWISS:
P55008

Gene ID:
199

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
?
產品圖片

Sample:
Lane 1: Lung (Rat) Lysate at 40 ug
Lane 2: Lung (Mouse) Lysate at 40 ug
Lane 3: Spleen (Rat) Lysate at 40 ug
Lane 4: Spleen (Mouse) Lysate at 40 ug
Lane 5: Lymph node (Rat) Lysate at 40 ug
Lane 6: Lymph node (Mouse) Lysate at 40 ug
Lane 7: Bone (Rat) Lysate at 40 ug
Lane 8: Bone (Mouse) Lysate at 40 ug
Lane 9: Cerebrum (Rat) Lysate at 40 ug
Lane 10: U937 (Human) Cell Lysate at 30 ug
Primary: Anti-AIF1 (bsm-54132R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 17 kD


Sample:
Lane 1: U937 (Human) Cell Lysate at 30 ug
Lane 2: Spleen (Mouse) Lysate at 40 ug
Lane 3: Spleen (Rat) Lysate at 40 ug
Lane 4: Lymph node (Mouse) Lysate at 40 ug
Lane 5: Lymph node (Rat) Lysate at 40 ug
Lane 6: Bone (Mouse) Lysate at 40 ug
Lane 7: Bone (Rat) Lysate at 40 ug
Lane 8: Cerebrum (Mouse) Lysate at 40 ug
Lane 9: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-AIF1 (bsm-54132R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 15 kD
?
?